OBERSTRASS, Florian, Albert LEE, Richard ŠTEFL, Michael JANIS, Guillaume CHANFREAU a Frederic ALLAIN. Shape-specific recognition in the structure of the Vts1p SAM domain with RNA. NATURE STRUCTURAL & MOLECULAR BIOLOGY. 2006, roč. 13, č. 2, s. 160-167. ISSN 1545-9985. |
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@article{710501, author = {Oberstrass, Florian and Lee, Albert and Štefl, Richard and Janis, Michael and Chanfreau, Guillaume and Allain, Frederic}, article_number = {2}, keywords = {SACCHAROMYCES-CEREVISIAE; REPRESSES TRANSLATION; DEADENYLASE COMPLEX; DROSOPHILA EMBRYO; DIPOLAR COUPLINGS; CRYSTAL-STRUCTURE; CCR4-NOT COMPLEX; GENE-EXPRESSION}, language = {eng}, issn = {1545-9985}, journal = {NATURE STRUCTURAL & MOLECULAR BIOLOGY}, title = {Shape-specific recognition in the structure of the Vts1p SAM domain with RNA}, volume = {13}, year = {2006} }
TY - JOUR ID - 710501 AU - Oberstrass, Florian - Lee, Albert - Štefl, Richard - Janis, Michael - Chanfreau, Guillaume - Allain, Frederic PY - 2006 TI - Shape-specific recognition in the structure of the Vts1p SAM domain with RNA JF - NATURE STRUCTURAL & MOLECULAR BIOLOGY VL - 13 IS - 2 SP - 160-167 EP - 160-167 SN - 15459985 KW - SACCHAROMYCES-CEREVISIAE KW - REPRESSES TRANSLATION KW - DEADENYLASE COMPLEX KW - DROSOPHILA EMBRYO KW - DIPOLAR COUPLINGS KW - CRYSTAL-STRUCTURE KW - CCR4-NOT COMPLEX KW - GENE-EXPRESSION N2 - Although the abundant sterile alpha motif (SAM) domain was originally classified as a protein-protein interaction domain, it has recently been shown that certain SAM domains have the ability to bind RNA, defining a new type of post-transcriptional gene regulator. To further understand the function of SAM-RNA recognition, we determined the solution structures of the SAM domain of the Saccharomyces cerevisiae Vts1p (Vts1p-SAM) and the Smaug response element (SRE) stem-loop RNA as a complex and in isolation. The structures show that Vts1p-SAM recognizes predominantly the shape of the SRE rather than its sequence, with the exception of a G located at the tip of the pentaloop. Using microarray gene profiling, we identified several genes in S. cerevisiae that seem to be regulated by Vts1p and contain one or more copies of the SRE. ER -
OBERSTRASS, Florian, Albert LEE, Richard ŠTEFL, Michael JANIS, Guillaume CHANFREAU a Frederic ALLAIN. Shape-specific recognition in the structure of the Vts1p SAM domain with RNA. \textit{NATURE STRUCTURAL \&{} MOLECULAR BIOLOGY}. 2006, roč.~13, č.~2, s.~160-167. ISSN~1545-9985.
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