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@article{710590,
author = {Hasoň, Stanislav and Vetterl, Vladimír},
article_location = {Amsterdam},
article_number = {3},
keywords = {gold amalgam-alloy electrode; platinum amalgam-alloy electrode; silver amalgam-alloy electrode; copper amalgam-alloy electrode; oligodeoxynucleotides; ODN; purine base residue-copper(I) complex; analysis of microliter volumes of ODN; ODN adsorption},
language = {eng},
issn = {0039-9140},
journal = {Talanta},
title = {Microanalysis of oligodeoxynucleotides by cathodic stripping voltammetry at amalgam-alloy surfaces in the presence of copper ions.},
volume = {69},
year = {2006}
}
TY - JOUR
ID - 710590
AU - Hasoň, Stanislav - Vetterl, Vladimír
PY - 2006
TI - Microanalysis of oligodeoxynucleotides by cathodic stripping voltammetry at amalgam-alloy surfaces in the presence of copper ions.
JF - Talanta
VL - 69
IS - 3
SP - 572-580
EP - 572-580
PB - Elsevier Science
SN - 00399140
KW - gold amalgam-alloy electrode
KW - platinum amalgam-alloy electrode
KW - silver amalgam-alloy electrode
KW - copper amalgam-alloy electrode
KW - oligodeoxynucleotides
KW - ODN
KW - purine base residue-copper(I) complex
KW - analysis of microliter volumes of ODN
KW - ODN adsorption
N2 - The application of gold amalgam-alloy electrode (AuAE) for a sensitive voltammetric detection of different oligodeoxynucleotides (ODNs) containing the purine units within the ODN-chains in the presence of copper is described. The detection of ODNs is based on the following steps: (i)an acidic hydrolysis of the ODN (ahODN) samples performing the release of the purine bases from ODN-chain; (ii)an electrochemical accumulation of the complex of the purine base residues released from ODN-chain with copper ions Cu(I) (ahODN-Cu(I) complex) at the potential of reduction of copper ions Cu(II) on the amalgam-alloy electrode surfaces; (iii) finally followed the cathodic stripping of the electrochemically accumulated ahODN-Cu(I) complex from the electrode surface. The proposed electrochemical method was used for: (a) detection of different ODN lengths containing only adenine units (the number of adenine units within the ODN-chains was changed from 10 to 80), and (b) determination of the number of purine units within the 30-mer ODNs containing a random sequence segments involving both the purine and pyrimidine units.
ER -
HASOŇ, Stanislav and Vladimír VETTERL. Microanalysis of oligodeoxynucleotides by cathodic stripping voltammetry at amalgam-alloy surfaces in the presence of copper ions. \textit{Talanta}. Amsterdam: Elsevier Science, 2006, vol.~69, No~3, p.~572-580. ISSN~0039-9140.
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