Flow injection analysis coupled with carbon electrodes as the
tool for analysis of naphthoquinones with respect to their
content and functions in biological samples

J 2006

Flow injection analysis coupled with carbon electrodes as the tool for analysis of naphthoquinones with respect to their content and functions in biological samples

BABULA, P., D. HUSKA, P. HANUŠTIAK, J. BALOUN, Soňa KŘÍŽKOVÁ et. al.

Základní údaje

Originální název

Flow injection analysis coupled with carbon electrodes as the tool for analysis of naphthoquinones with respect to their content and functions in biological samples

Název česky

Průtoková injekční analýzy ve spojení s uhlíkovou elektrodou jako nástroj pro analýzu naftochinonů s ohledem na obsah a funkce v biologickém materiálu

Autoři

BABULA, P. (203 Česká republika), D. HUSKA (203 Česká republika), P. HANUŠTIAK (203 Česká republika), J. BALOUN (203 Česká republika), Soňa KŘÍŽKOVÁ (203 Česká republika), Vojtěch ADAM (203 Česká republika), J. HUBÁLEK (203 Česká republika), L. HAVEL (203 Česká republika), M. ŽEMLIČKA (203 Česká republika), Aleš HORNA (203 Česká republika), Miroslava BEKLOVÁ (203 Česká republika) a René KIZEK (203 Česká republika, garant)

Vydání

SENSORS, 2006, 1424-8220

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.373

Kód RIV

RIV/00216224:14310/06:00016397

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000244633800003

Klíčová slova anglicky

Flow injection analysis; carbon electrodes; naphthoquinones; biological samples

Štítky

biological samples, carbon electrodes, Flow injection analysis, naphthoquinones

Změněno: 14. 2. 2007 17:50, Ing. Radka Mikelová, Ph.D.

Anotace

ORIG CZ

V originále

Naphthoquinones are one of the groups of secondary metabolites widespread in nature, where they mostly appear as chromatic pigments. They embody broad-range of biological actions from phytotoxic to fungicidal. An anticancer effect of naphthoquinones stimulates an interest in determination and characterization of single derivatives of 1,2- and 1,4-quinones in biological samples. The main aim of this work was to suggest a technique suitable to determine lawsone, juglone and/or plumbagin in biological samples and to study of their influence on BY-2 tobacco cells. The BY-2 tobacco cells were cultivated in the presence of the naphthoquinones of interest (500 ug.l-1) for 24 h and then the morphological changes were observed. We found out that naphthoquinones triggered the programmed cell death at BY-2 cells, which can be confirmed by the apoptotic bodies in nucleus. After that we suggested and optimized different electrochemical techniques such differential pulse voltammetry (DPV) coupled with hanging mercury drop (HMDE) and carbon paste electrode, micro flow device coupled with carbon screen printed electrodes and flow injection analysis coupled with Coulochem III detector to determine them. The detection limits of naphthoquinones of interest were expressed as 3S/N and varied from units to hundreds of ng per millilitres according to methods used. Moreover, we utilized DPV coupled with HMDE and micro flow device to determine content of juglone in leaves Persian walnut (Juglans regia). We determined that the leaves contained juglone tenths of g per 100 g of fresh weight. The results obtained show the convincing possibilities of using of these methods in analysis of plant secondary metabolites.

Česky

Průtoková injekční analýzy ve spojení s uhlíkovou elektrodou jako nástroj pro analýzu naftochinonů s ohledem na obsah a funkce v biologickém materiálu

Návaznosti

GP525/04/P132, projekt VaV

Název: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

Citovat

BABULA, P., D. HUSKA, P. HANUŠTIAK, J. BALOUN, Soňa KŘÍŽKOVÁ, Vojtěch ADAM, J. HUBÁLEK, L. HAVEL, M. ŽEMLIČKA, Aleš HORNA, Miroslava BEKLOVÁ a René KIZEK. Flow injection analysis coupled with carbon electrodes as the tool for analysis of naphthoquinones with respect to their content and functions in biological samples. SENSORS. 2006, roč. 11, č. 6, s. 1466-1482. ISSN 1424-8220.

@article{712566,
   author = {Babula, P. and Huska, D. and Hanuštiak, P. and Baloun, J. and Křížková, Soňa and Adam, Vojtěch and Hubálek, J. and Havel, L. and Žemlička, M. and Horna, Aleš and Beklová, Miroslava and Kizek, René},
   article_number = {6},
   keywords = {Flow injection analysis; carbon electrodes; naphthoquinones; biological samples},
   language = {eng},
   issn = {1424-8220},
   journal = {SENSORS},
   title = {Flow injection analysis coupled with carbon electrodes as the tool for analysis of naphthoquinones with respect to their content and functions in biological samples},
   volume = {11},
   year = {2006}
}

TY  - JOUR
ID  - 712566
AU  - Babula, P. - Huska, D. - Hanuštiak, P. - Baloun, J. - Křížková, Soňa - Adam, Vojtěch - Hubálek, J. - Havel, L. - Žemlička, M. - Horna, Aleš - Beklová, Miroslava - Kizek, René
PY  - 2006
TI  - Flow injection analysis coupled with carbon electrodes as the tool for analysis of naphthoquinones with respect to their content and functions in biological samples
JF  - SENSORS
VL  - 11
IS  - 6
SP  - 1466-1482
EP  - 1466-1482
SN  - 14248220
KW  - Flow injection analysis
KW  - carbon electrodes
KW  - naphthoquinones
KW  - biological samples
N2  - Naphthoquinones are one of the groups of secondary metabolites widespread in nature, where they mostly appear as chromatic pigments. They embody broad-range of biological actions from phytotoxic to fungicidal. An anticancer effect of naphthoquinones stimulates an interest in determination and characterization of single derivatives of 1,2- and 1,4-quinones in biological samples. The main aim of this work was to suggest a technique suitable to determine lawsone, juglone and/or plumbagin in biological samples and to study of their influence on BY-2 tobacco cells. The BY-2 tobacco cells were cultivated in the presence of the naphthoquinones of interest (500 ug.l-1) for 24 h and then the morphological changes were observed. We found out that naphthoquinones triggered the programmed cell death at BY-2 cells, which can be confirmed by the apoptotic bodies in nucleus. After that we suggested and optimized different electrochemical techniques such differential pulse voltammetry (DPV) coupled with hanging mercury drop (HMDE) and carbon paste electrode, micro flow device coupled with carbon screen printed electrodes and flow injection analysis coupled with Coulochem III detector to determine them. The detection limits of naphthoquinones of interest were expressed as 3S/N and varied from units to hundreds of ng per millilitres according to methods used. Moreover, we utilized DPV coupled with HMDE and micro flow device to determine content of juglone in leaves Persian walnut (Juglans regia). We determined that the leaves contained juglone tenths of g per 100 g of fresh weight. The results obtained show the convincing possibilities of using of these methods in analysis of plant secondary metabolites.
ER  -

BABULA, P., D. HUSKA, P. HANUŠTIAK, J. BALOUN, Soňa KŘÍŽKOVÁ, Vojtěch ADAM, J. HUBÁLEK, L. HAVEL, M. ŽEMLIČKA, Aleš HORNA, Miroslava BEKLOVÁ a René KIZEK. Flow injection analysis coupled with carbon electrodes as the tool for analysis of naphthoquinones with respect to their content and functions in biological samples. \textit{SENSORS}. 2006, roč.~11, č.~6, s.~1466-1482. ISSN~1424-8220.

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