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@article{712640,
author = {Mikelová, Radka and Trnková, Libuše and Jelen, František and Adam, Vojtěch and Kizek, René},
article_location = {Weinheim},
article_number = {2-3},
keywords = {reduction signals;short hetero-oligonucleotides; elimination voltammetry},
language = {eng},
issn = {1040-0397},
journal = {Electroanalysis},
title = {Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry},
volume = {19},
year = {2007}
}
TY - JOUR
ID - 712640
AU - Mikelová, Radka - Trnková, Libuše - Jelen, František - Adam, Vojtěch - Kizek, René
PY - 2007
TI - Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry
JF - Electroanalysis
VL - 19
IS - 2-3
SP - 348-355
EP - 348-355
PB - Wiley.VCH Verlag
SN - 10400397
KW - reduction signals;short hetero-oligonucleotides
KW - elimination voltammetry
N2 - The resolution of overlapped reduction voltammetric signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C, but with the same ratio (A/C = 1/3), was carried out by elimination voltammetry with linear scan (EVLS). The EVLS, as a mathematical transformation of voltammetric total currents measured at different scan rates, is capable to yield very sensitive resolution of potentially closed signals, which cannot be separated by common electrochemical methods. The best resolution of A and C signals was observed when the EVLS function eliminating the charging and kinetic currents and conserving the diffusion current was combined with adsorption stripping technique (AdS). The resulting peak-counterpeak signal made it possible not only to separate the responses of A and C residues, but also to follow the dynamics of adsorption processes on mercury electrode surfaces. Together with the effect of the accumulation time on peak-counterpeak signal, the effect of pH and temperature on this signal was studied. The relation between elimination signals and the structure changes of ODNs was discussed.
ER -
MIKELOVÁ, Radka, Libuše TRNKOVÁ, František JELEN, Vojtěch ADAM a René KIZEK. Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry. \textit{Electroanalysis}. Weinheim: Wiley.VCH Verlag, 2007, roč.~19, 2-3, s.~348-355. ISSN~1040-0397.
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