HPLC determination of midazolam and its three hydroxy
metabolites in perfusion medium and plasma from rats

J 2007

HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats

JUŘICA, Jan, Miroslav DOSTÁLEK, Jiří KONEČNÝ, Zdeněk GLATZ, Eva HADAŠOVÁ et. al.

Basic information

Original name

HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats

Name in Czech

HPLC stanovení midazolamu a jeho tří hydroxy-metabolitů v perfuzním médiu a plazmě potkanů

Authors

JUŘICA, Jan (203 Czech Republic, belonging to the institution), Miroslav DOSTÁLEK (203 Czech Republic), Jiří KONEČNÝ (203 Czech Republic, belonging to the institution), Zdeněk GLATZ (203 Czech Republic, belonging to the institution), Eva HADAŠOVÁ (203 Czech Republic, belonging to the institution) and Josef TOMANDL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Chromatography B, Amsterdam, The Netherlands, Elsevier, 2007, 1570-0232

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.935

RIV identification code

RIV/00216224:14310/07:00020174

Organization unit

Faculty of Science

UT WoS

000247286700078

Keywords in English

cytochrome P450; midazolam; 1'-hydroxymidazolam; 4-hydroxymidazolam; 1'4-dihydroxymidazolam; rat; perfusion; plasma; HPLC

Abstract

ORIG CZ

V originále

A new, simple, rapid, sensitive, and repeatable isocratic reverse-phase HPLC method was developed and validated for simultaneous determination of midazolam and its main three hydroxylated metabolites, i.e. 1' hydroxymidazolam, 4-hydroxymidazolam, and 1',4-dihydroxymidazolam in rat liver perfusate and also plasma. Diazepam was used as an internal standard to ensure precision and accuracy of this method. This method is applicable for modeling and description of possible pharmacological interactions on rat (CYP3A1/2) or human (CYP3A4/5) cytochrome P450 enzymes.

In Czech

Byla vypracována HPLC metoad současného stanovení midazolamu a jeho hlavních tří hydroxylovaných metabolitů v perfuzním mediu a plazmě ptokanů. Metoda je využitelná při modelování a charakterizaci možných farmakologických interakcí isoformy CYP3A1/2 u potkanů nebo CYP3A4/5 u člověka.

Links

GA203/06/0047, research and development project

Name: Využití kapilární elektroforézy pro studium metabolismu léčiv

Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies

LC06023, research and development project

Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie

Investor: Ministry of Education, Youth and Sports of the CR

MSM0021622404, plan (intention)

Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.

Citovat

JUŘICA, Jan, Miroslav DOSTÁLEK, Jiří KONEČNÝ, Zdeněk GLATZ, Eva HADAŠOVÁ and Josef TOMANDL. HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats. Journal of Chromatography B. Amsterdam, The Netherlands: Elsevier, 2007, vol. 852, 1-2, p. 571-577. ISSN 1570-0232.

@article{713129,
   author = {Juřica, Jan and Dostálek, Miroslav and Konečný, Jiří and Glatz, Zdeněk and Hadašová, Eva and Tomandl, Josef},
   article_location = {Amsterdam, The Netherlands},
   article_number = {1-2},
   keywords = {cytochrome P450; midazolam; 1'-hydroxymidazolam; 4-hydroxymidazolam; 1'4-dihydroxymidazolam; rat; perfusion; plasma; HPLC},
   language = {eng},
   issn = {1570-0232},
   journal = {Journal of Chromatography B},
   note = {doi:10.1016/j.jchromb.2007.02.034},
   title = {HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats},
   url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6X0P-4N4J320-4&_user=835458&_coverDate=06%2F01%2F2007&_alid=584152625&_rdoc=1&_fmt=summary&_orig=search&_cdi=7220&_sort=d&_st=4&_docanchor=&_ct=44&_acct=C000045159&_version=1&_urlVersion=0&_userid},
   volume = {852},
   year = {2007}
}

TY  - JOUR
ID  - 713129
AU  - Juřica, Jan - Dostálek, Miroslav - Konečný, Jiří - Glatz, Zdeněk - Hadašová, Eva - Tomandl, Josef
PY  - 2007
TI  - HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats
JF  - Journal of Chromatography B
VL  - 852
IS  - 1-2
SP  - 571-577
EP  - 571-577
PB  - Elsevier
SN  - 15700232
N1  - doi:10.1016/j.jchromb.2007.02.034
KW  - cytochrome P450
KW  - midazolam
KW  - 1'-hydroxymidazolam
KW  - 4-hydroxymidazolam
KW  - 1'4-dihydroxymidazolam
KW  - rat
KW  - perfusion
KW  - plasma
KW  - HPLC
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6X0P-4N4J320-4&_user=835458&_coverDate=06%2F01%2F2007&_alid=584152625&_rdoc=1&_fmt=summary&_orig=search&_cdi=7220&_sort=d&_st=4&_docanchor=&_ct=44&_acct=C000045159&_version=1&_urlVersion=0&_userid
N2  - A new, simple, rapid, sensitive, and repeatable isocratic reverse-phase HPLC method was developed and validated for simultaneous determination of midazolam and its main three hydroxylated metabolites, i.e. 1' hydroxymidazolam, 4-hydroxymidazolam, and 1',4-dihydroxymidazolam in rat liver perfusate and also plasma. Diazepam was used as an internal standard to ensure precision and accuracy of this method. This method is applicable for modeling and description of possible pharmacological interactions on rat (CYP3A1/2) or human (CYP3A4/5) cytochrome P450 enzymes.
ER  -

JUŘICA, Jan, Miroslav DOSTÁLEK, Jiří KONEČNÝ, Zdeněk GLATZ, Eva HADAŠOVÁ and Josef TOMANDL. HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats. \textit{Journal of Chromatography B}. Amsterdam, The Netherlands: Elsevier, 2007, vol.~852, 1-2, p.~571-577. ISSN~1570-0232.

Detailed Information on Publication Record