The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella

HYRŠL, Pavel, Ender BÜYÜKGÜZEL and Kemal BÜYÜKGÜZEL. The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella. Archives of Insect Biochemistry and Physiology. Wiley, 2007, vol. 66, No 1, p. 23-31. ISSN 0739-4462.

Basic information

• Original name: The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella
• Name in Czech: Vliv oxidativního stresu indukovaného kyselinou boritou na antioxidační enzymy a přežívání Galleria mellonella
• Authors: HYRŠL, Pavel (203 Czech Republic, guarantor), Ender BÜYÜKGÜZEL (792 Turkey) and Kemal BÜYÜKGÜZEL (792 Turkey).
• Edition: Archives of Insect Biochemistry and Physiology, Wiley, 2007, 0739-4462.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 30105 Physiology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.345
• RIV identification code: RIV/00216224:14310/07:00031927
• Organization unit: Faculty of Science
• UT WoS: 000248872200003
• Keywords in English: Galleria mellonella; boric acid; antioxidant enzymes; hemolymph; fat body; malondialdehyde; survivorship
• Tags: antioxidant enzymes, boric acid, fat body, Galleria mellonella, hemolymph, malondialdehyde, survivorship
• Tags: International impact, Reviewed

Abstract

Larvae of the wax moth, Galleria mellonella (L.), were reared from first instar on a diet supplemented with 156, 620, 1250, or 2500 ppm boric acid (BA). The content of malondialdehyde (MDA, an oxidative stress indicator), and activities of the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and glutathione peroxidase (GPx)] were determined in the fat body and hemolymph in the 7th instar larvae and newly emerged pupae. Relative to control larvae, MDA was significantly increased in larval hemolymph, larval and pupal fat body, but decreased in the pupal hemolymph. Insects reared on diets with 156 and 620 ppm BA doses yielded increased SOD activity but 1250 and 2500 ppm doses resulted in decreased SOD activity in larval hemolymph. SOD activity was significantly increased but CAT was decreased in the larval fat body. High dietary BA treatments led to significantly decreased GST activity, however they increased GPx activity in larval hemolymph. Dietary BA also affected larval survival. The 1250 and 2500 ppm concentrations led to significantly increased larval and pupal mortality and prolonged development. By contrast, the lowest BA concentration increased longevity and shortened development. We infer that BA toxicity is related, at least in part, to oxidative stress management.

Abstract (in Czech)

Larvae of the wax moth, Galleria mellonella (L.), were reared from first instar on a diet supplemented with 156, 620, 1250, or 2500 ppm boric acid (BA). The content of malondialdehyde (MDA, an oxidative stress indicator), and activities of the antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and glutathione peroxidase (GPx)] were determined in the fat body and hemolymph in the 7th instar larvae and newly emerged pupae. Relative to control larvae, MDA was significantly increased in larval hemolymph, larval and pupal fat body, but decreased in the pupal hemolymph. Insects reared on diets with 156 and 620 ppm BA doses yielded increased SOD activity but 1250 and 2500 ppm doses resulted in decreased SOD activity in larval hemolymph. SOD activity was significantly increased but CAT was decreased in the larval fat body. High dietary BA treatments led to significantly decreased GST activity, however they increased GPx activity in larval hemolymph. Dietary BA also affected larval survival. The 1250 and 2500 ppm concentrations led to significantly increased larval and pupal mortality and prolonged development. By contrast, the lowest BA concentration increased longevity and shortened development. We infer that BA toxicity is related, at least in part, to oxidative stress management.