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@inproceedings{713748,
author = {Jungmannová, Barbara and Řepková, Jana},
address = {Perugia, Italy},
booktitle = {Breeding and seed production for conventional and organic agriculture},
keywords = {Lotus - interspecific hybridization - prezygotic barriers - postzygotic barriers},
language = {eng},
location = {Perugia, Italy},
isbn = {978-88-87652-12-3},
pages = {341-343},
publisher = {University of Perugia},
title = {Study of prezygotic and postzygotic barriers of crossability in the genus Lotus.},
year = {2007}
}
TY - JOUR
ID - 713748
AU - Jungmannová, Barbara - Řepková, Jana
PY - 2007
TI - Study of prezygotic and postzygotic barriers of crossability in the genus Lotus.
PB - University of Perugia
CY - Perugia, Italy
SN - 9788887652123
KW - Lotus - interspecific hybridization - prezygotic barriers - postzygotic barriers
N2 - Lotus corniculatus L. is a crucial component of meadow and pasture vegetation and can be used as a fodder crop. Its growth is limited by differences in maturation and pod shattering, which also affect the seed yield. Shattering resistance is a characteristic of high heritability and, in Lotus, is considered to be controlled by more than one gene. Classical breeding approaches to reduce shattering through recurrent selection were unsuccessful. The problem of pod shattering can be solved by means of interspecific hybridization with various distantly related indehiscent Lotus species. Our work was aimed at the study of prezygotic and postzygotic barriers of crossability between L. corniculatus and sources of the indehiscent seed pod trait, such as L. uliginosus (2n=2x and 4x). The growth of the pollen tubes up to the ovary was observed after the crosses of L. corniculatus x L. uliginosus (2n=2x and 4x). This means that prezygotic barriers were not detected in this combination. In L. corniculatus, L. uliginosus (2x, 4x), the method of clearing treatment of plant tissues by chloral hydrate was used. Clearing of immature seeds and inner embryos up to heart stage was carried out after modifications of a known procedure. The optimal length of fixation was 24 h and the optimal length of action of chloral hydrate was 48 h. For other embryo stages, this method has not been sufficiently optimised so far. Even if enlargement of immature seeds occurred, postzygotic barriers after interspecific crosses of L. corniculatus x L. uliginosus (2x) covered defects of embryo sac and no hybrid embryo was traced. In the interspecific combination of L. corniculatus x L. uliginosus (4x), globular stage embryo was detected.
ER -
JUNGMANNOVÁ, Barbara a Jana ŘEPKOVÁ. Study of prezygotic and postzygotic barriers of crossability in the genus Lotus. In \textit{Breeding and seed production for conventional and organic agriculture}. Perugia, Italy: University of Perugia, 2007, s.~341-343. ISBN~978-88-87652-12-3.
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