2007
Distinct patterns of histone methylation and acetylation in human interphase nuclei
SKALNÍKOVÁ, Magdalena, Eva BÁRTOVÁ, Vladimír ULMAN, Petr MATULA, David SVOBODA et. al.Základní údaje
Originální název
Distinct patterns of histone methylation and acetylation in human interphase nuclei
Název česky
Rozdílné paterny metylace a acetylace histonů v lidských interfázních jádrech
Autoři
SKALNÍKOVÁ, Magdalena (203 Česká republika), Eva BÁRTOVÁ (203 Česká republika), Vladimír ULMAN (203 Česká republika, domácí), Petr MATULA (203 Česká republika, domácí), David SVOBODA (203 Česká republika, domácí), Andrea HARNIČAROVÁ (703 Slovensko, domácí), Michal KOZUBEK (203 Česká republika, garant, domácí) a Stanislav KOZUBEK (203 Česká republika, domácí)
Vydání
Physiological Research, Prague, Czech Academy of Sciences, 2007, 0862-8408
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.505
Kód RIV
RIV/00216224:14330/07:00020185
Organizační jednotka
Fakulta informatiky
UT WoS
000252358700017
Klíčová slova anglicky
Histone methylation; acetylation; X chromosome; centromere; image analysis
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 20. 6. 2012 09:03, RNDr. Vladimír Ulman, Ph.D.
V originále
To study 3D nuclear distributions of epigenetic histone modifications such as H3(K9) acetylation, H3(K4) dimethylation, H3(K9) dimethylation, and H3(K27) trimethylation, and of histone methyl transferase Suv39H1, we used advanced image analysis methods, combined with Nipkow disk confocal microscopy. Total fluorescence intensity and distributions of fluorescently labelled proteins were analysed in formaldehyde-fixed interphase nuclei. Our data showed reduced fluorescent signals of H3(K9) acetylation and H3(K4) dimethylation (dime) at the nuclear periphery, while di-meH3(K9) was also abundant in chromatin regions closely associated with the nuclear envelope. Little overlapping (intermingling) was observed for di-meH3(K4) and H3(K27) trimethylation (tri-me), and for di-meH3(K9) and Suv39H1. The histone modifications studied were absent in the nucleolar compartment with an exception of H3(K9) dimethylation that was closely associated to perinucleolar regions which are formed by centromeres of acrocentric chromosomes. Using immunocytochemistry, no dimeH3( K4) but just dense di-meH3(K9) was found for the human acrocentric chromosomes 14 and 22. The active X chromosome was observed to be partially acetylated, while the inactive X was more condensed, located in a very peripheral part of the interphase nuclei, and lacked H3(K9) acetylation. Our results confirmed specific interphase patterns of histone modifications within the interphase nuclei as well as within their chromosome territories.
Česky
Práce se zabývá různými "paterny" metylace a acetylace histonů v lidských interfázních jádrech
Návaznosti
| GP202/03/D033, projekt VaV |
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| LC535, projekt VaV |
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| MSM0021622419, záměr |
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