J 2007

Experimental design in intraspecific organelle DNA sequence studies I: haplotype detection methods

STONEBERG HOLT, Sierra Dawn and Petr BUREŠ

Basic information

Original name

Experimental design in intraspecific organelle DNA sequence studies I: haplotype detection methods

Name in Czech

Experimentální design intraspecifických studií založených na studiu sekvencí organelové DNA I: metody detekce haplotypů

Authors

STONEBERG HOLT, Sierra Dawn (840 United States of America) and Petr BUREŠ (203 Czech Republic, guarantor)

Edition

Taxon, International Assoc. for Plant Taxonomy, 2007, 0040-0262

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.524

RIV identification code

RIV/00216224:14310/07:00022014

Organization unit

Faculty of Science

UT WoS

000244825300015

Keywords in English

chemical cleavage of mismatch (CCM); heteroduplex analysis (HA); intraspecific organelle sequence studies; literature review; phylogeography; single-strand conformation polymorphism (SSCP)

Tags

International impact, Reviewed
Změněno: 25/6/2009 11:09, prof. RNDr. Petr Bureš, Ph.D.

Abstract

V originále

Phylogenetic studies of organelle DNA sequences at low taxonomic levels present a researcher with specific experimental design challenges. Such studies require sampling of large numbers of individuals, many of which share identical haplotypes. The process is often limited by time and money required for DNA sequencing. A variety of mutation detection methods has been developed to circumvent this limitation. This paper briefly reviews the available methods and presents some of their advantages and disadvantages. The applicability of these methods to nuclear DNA studies is discussed.

In Czech

Přehled metod užívaných k detekci haplotypů

Links

LC06073, research and development project
Name: Centrum pro výzkum biodiverzity
Investor: Ministry of Education, Youth and Sports of the CR, Biodiversity Research Center
MSM0021622416, plan (intention)
Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time