| NEČESALOVÁ, Eva, Vladimíra VRANOVÁ, Petr KUGLÍK, Pavel CEJPEK, Marie JAROŠOVÁ, Martina PEŠÁKOVÁ, Jiřina RELICHOVÁ a Renata VESELSKÁ. Incidence of the main genetic markers in glioblastoma multiforme is independent of tumor topology. Neoplasma. 2007, roč. 54, č. 3, s. 212-218. ISSN 0028-2685. |
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@article{718463,
author = {Nečesalová, Eva and Vranová, Vladimíra and Kuglík, Petr and Cejpek, Pavel and Jarošová, Marie and Pešáková, Martina and Relichová, Jiřina and Veselská, Renata},
article_number = {3},
keywords = {glioblastoma multiforme; molecular cytogenetics; FISH; array-CGH},
language = {eng},
issn = {0028-2685},
journal = {Neoplasma},
title = {Incidence of the main genetic markers in glioblastoma multiforme is independent of tumor topology},
volume = {54},
year = {2007}
}
TY - JOUR
ID - 718463
AU - Nečesalová, Eva - Vranová, Vladimíra - Kuglík, Petr - Cejpek, Pavel - Jarošová, Marie - Pešáková, Martina - Relichová, Jiřina - Veselská, Renata
PY - 2007
TI - Incidence of the main genetic markers in glioblastoma multiforme is independent of tumor topology
JF - Neoplasma
VL - 54
IS - 3
SP - 212-218
EP - 212-218
SN - 00282685
KW - glioblastoma multiforme
KW - molecular cytogenetics
KW - FISH
KW - array-CGH
N2 - Glioblastoma multiforme (GBM) is the most common as well as the most aggressive type of adult primary brain tumor of astrocytic origin. GBM is characterized by a high degree of intratumoral heterogeneity both in histomorphology and genetic changes. Nevertheless, trisomy/polysomy of chromosome 7, monosomy of chromosome 10, EGFR gene amplification and p53 deletion have been described as the typical genetic markers for tumor classification and prediction of possible response to therapy. Our work was based on detection of these four main genetic changes both in central and peripheral parts of the tumors to evaluate possible differences in the topological incidence of these genetic markers. Chromosomal abnormalities in tumor samples from a group of 21 patients surgically treated for GBM were characterized by means of the interphase-fluorescence in situ hybridization (I-FISH) technique using sets of centromere and locus-specific DNA probes. In addition, we performed a detailed analysis of one selected tumor sample using a genomic microarray system (GenoSensor Array 300) to characterize copy number changes of specific sequences and refine results obtained by I-FISH. However, the data show no significant differences in occurrence of the described genetic markers in either part of the tumors.
ER -
NEČESALOVÁ, Eva, Vladimíra VRANOVÁ, Petr KUGLÍK, Pavel CEJPEK, Marie JAROŠOVÁ, Martina PEŠÁKOVÁ, Jiřina RELICHOVÁ a Renata VESELSKÁ. Incidence of the main genetic markers in glioblastoma multiforme is independent of tumor topology. \textit{Neoplasma}. 2007, roč.~54, č.~3, s.~212-218. ISSN~0028-2685.
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