Application of fluorimetric analysis of plant esterases to
study of programmed cell death and effects of cadmium(II) ions

J 2007

Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions

VÍTEČEK, Jan, Jitka PETRLOVÁ, Jiří PETŘEK, Vojtěch ADAM, L. HAVEL et. al.

Základní údaje

Originální název

Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions

Název česky

Aplikace fluorimetrické analýzy rostlinných esteráz při studiu progamované buněčné smrti a vliv kademnatých iontů

Autoři

VÍTEČEK, Jan (203 Česká republika), Jitka PETRLOVÁ (203 Česká republika), Jiří PETŘEK (203 Česká republika), Vojtěch ADAM (203 Česká republika), L. HAVEL (203 Česká republika), K.J. KRAMER (840 Spojené státy) a René KIZEK (203 Česká republika, garant)

Vydání

Biologica Plantarum, 2007, 0006-3134

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.259

Kód RIV

RIV/00216224:14310/07:00020262

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000247412400026

Klíčová slova anglicky

fluorimetric analysis; plant esterases; programmed cell death; cadmium(II) ions

Štítky

cadmium(II) ions, fluorimetric analysis, plant esterases, programmed cell death

Příznaky

Recenzováno

Změněno: 29. 5. 2007 10:14, Ing. Radka Mikelová, Ph.D.

Anotace

ORIG CZ

V originále

Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of carboxyl ester bonds. Their connection with development has made them a suitable marker of development in plants. In the present work, we focused on the fluorimetric determination of the plant esterases in plant cell cultures (tobacco BY-2 cells and early somatic embryos of Norway spruce, clone 2/32) with respect to application the method for the study of programmed cell death and the influence of cadmium(II) ions on the plant cells. The programmed cell death has been triggered by sodium nitroprusside and glucose oxidase. The determination of the esterase activity by the proposed technique in a cell extract determined very small difference in enzyme activity, which was a reliable marker of metabolic changes. In addition, the esterase activity of spruce somatic embryos decreased with the increase in medium Cd concentration.

Česky

Aplikace fluorimetrické analýzy rostlinných esteráz při studiu progamované buněčné smrti a vliv kademnatých iontů

Návaznosti

GP525/04/P132, projekt VaV

Název: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

Citovat

VÍTEČEK, Jan, Jitka PETRLOVÁ, Jiří PETŘEK, Vojtěch ADAM, L. HAVEL, K.J. KRAMER a René KIZEK. Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions. Biologica Plantarum. 2007, roč. 51, č. 3, s. 551-555. ISSN 0006-3134.

@article{718616,
   author = {Víteček, Jan and Petrlová, Jitka and Petřek, Jiří and Adam, Vojtěch and Havel, L. and Kramer, K.J. and Kizek, René},
   article_number = {3},
   keywords = {fluorimetric analysis; plant esterases; programmed cell death; cadmium(II) ions},
   language = {eng},
   issn = {0006-3134},
   journal = {Biologica Plantarum},
   title = {Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions},
   volume = {51},
   year = {2007}
}

TY  - JOUR
ID  - 718616
AU  - Víteček, Jan - Petrlová, Jitka - Petřek, Jiří - Adam, Vojtěch - Havel, L. - Kramer, K.J. - Kizek, René
PY  - 2007
TI  - Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions
JF  - Biologica Plantarum
VL  - 51
IS  - 3
SP  - 551-555
EP  - 551-555
SN  - 00063134
KW  - fluorimetric analysis
KW  - plant esterases
KW  - programmed cell death
KW  - cadmium(II) ions
N2  - Esterases (EC 3.1.1.x) represent a diverse group of hydrolases catalyzing the cleavage and formation of carboxyl ester bonds. Their connection with development has made them a suitable marker of development in plants. In the present work, we focused on the fluorimetric determination of the plant esterases in plant cell cultures (tobacco BY-2 cells and early somatic embryos of Norway spruce, clone 2/32) with respect to application the method for the study of programmed cell death and the influence of cadmium(II) ions on the plant cells. The programmed cell death has been triggered by sodium nitroprusside and glucose oxidase. The determination of the esterase activity by the proposed technique in a cell extract determined very small difference in enzyme activity, which was a reliable marker of metabolic changes. In addition, the esterase activity of spruce somatic embryos decreased with the increase in medium Cd concentration.
ER  -

VÍTEČEK, Jan, Jitka PETRLOVÁ, Jiří PETŘEK, Vojtěch ADAM, L. HAVEL, K.J. KRAMER a René KIZEK. Application of fluorimetric analysis of plant esterases to study of programmed cell death and effects of cadmium(II) ions. \textit{Biologica Plantarum}. 2007, roč.~51, č.~3, s.~551-555. ISSN~0006-3134.

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