MIKELOVÁ, Radka, P. HODEK, Pavel HANUŠTIAK, Vojtěch ADAM, Soňa KŘÍŽKOVÁ, L. HAVEL, Marie STIBOROVÁ, A. HORNA, Miroslava BEKLOVÁ, Libuše TRNKOVÁ and René KIZEK. Utilizing of electrochemical detection coupled with liquid chromatography for isoflavones detection. Acta Chim. Slov. Ljubljana, Slovenia: Slovenian chemical society, 2007, vol. 54, No 1, p. 92-97. ISSN 1318-0207.
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Basic information
Original name Utilizing of electrochemical detection coupled with liquid chromatography for isoflavones detection
Name in Czech Využití elektrochemické detekce ve spojení s kapalinovou chromatografií pro detekci isoflanonů
Authors MIKELOVÁ, Radka (203 Czech Republic), P. HODEK (203 Czech Republic), Pavel HANUŠTIAK (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic), L. HAVEL (203 Czech Republic), Marie STIBOROVÁ (203 Czech Republic), A. HORNA (203 Czech Republic), Miroslava BEKLOVÁ (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor).
Edition Acta Chim. Slov. Ljubljana, Slovenia, Slovenian chemical society, 2007, 1318-0207.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 40106 Agronomy, plant breeding and plant protection;
Country of publisher Slovenia
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.093
RIV identification code RIV/00216224:14310/07:00020266
Organization unit Faculty of Science
Keywords in English electrochemical detection; liquid chromatography; isoflavones
Tags electrochemical detection, Isoflavones, liquid chromatography
Changed by Changed by: Ing. Radka Mikelová, Ph.D., učo 176915. Changed: 30/5/2007 08:06.
Abstract
Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L-1 acetate buffer (pH 5.0); flow rate 2.0 mL min-1; column and detector temperature: 26 C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL-1. Their simultaneous determination takes 15 min.
Abstract (in Czech)
Využití elektrochemické detekce ve spojení s kapalinovou chromatografií pro detekci isoflanonů
Links
GP525/04/P132, research and development projectName: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy
MSM0021622412, plan (intention)Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
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