Study of structural changes of lactoferrin using flow injection analysis with electrochemical detection on the surface of glassy carbon electrode

ZÍTKA, Ondřej, A. HORNA, Karel STEJSKAL, Josef ZEHNÁLEK, Vojtěch ADAM, L. HAVEL, L. ZEMAN and René KIZEK. Study of structural changes of lactoferrin using flow injection analysis with electrochemical detection on the surface of glassy carbon electrode. Acta Chim. Slov. 2007, vol. 54, No 1, p. 68-73. ISSN 1318-0207.

Basic information

Original name

Study of structural changes of lactoferrin using flow injection analysis with electrochemical detection on the surface of glassy carbon electrode

Name in Czech

Studium strukturních změn laktoferinu pomocí prutokové injekční analýzy s elektrochemickou detekcí na povrchu elektrody ze skelného uhlíku

Authors

ZÍTKA, Ondřej (203 Czech Republic), A. HORNA (203 Czech Republic), Karel STEJSKAL (203 Czech Republic), Josef ZEHNÁLEK (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), L. HAVEL (203 Czech Republic), L. ZEMAN (203 Czech Republic) and René KIZEK (203 Czech Republic, guarantor)

Edition

Acta Chim. Slov. 2007, 1318-0207

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40106 Agronomy, plant breeding and plant protection;

Country of publisher

Slovenia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.093

RIV identification code

RIV/00216224:14310/07:00022107

Organization unit

Faculty of Science

UT WoS

000245178800010

Keywords in English

structural changes; lactoferrin; flow injection analysis; electrochemical detection; glassy carbon electrode

Tags

electrochemical detection, Flow injection analysis, glassy carbon electrode, Lactoferrin, structural changes

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Abstract

V originále

Lactoferrin is considered to be a multifunctional protein. It appears to play several biologically important roles, where its structure is very crucial. The aim of this work was to investigate the basic electrochemical behaviour of lactoferrin by both stationary and flow electrochemical methods with respect its structural changes under various denaturing conditions. The electroactivity of lactoferrin was studied by three various electrochemical methods (linear sweep, differential pulse and square wave voltammetry). Based on the results obtained, we utilized flow injection analysis with electrochemical detection (FIA ED) for determination of lactoferrin. We found out that the most suitable FIA ED conditions were as follows: working electrode potential of 900 mV, Britton Robinson buffer (pH 4.5) as the mobile phase, its flow rate 1 ml min 1. Finally, we attempted to follow the changes of lactoferrin signal in the presence of chemical compounds or under the physical conditions leading to changes in its structure. As we have shown here, electrochemical analysis enables us to distinguish changes of protein structure easily and rapidly.

In Czech

Studium strukturních změn laktoferinu pomocí prutokové injekční analýzy s elektrochemickou detekcí na povrchu elektrody ze skelného uhlíku

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Links

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL) Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)