| ADAM, Jan, Martina POKORNÁ, Charles SABIN, Edward P. MITCHELL, Anne IMBERTY a Michaela WIMMEROVÁ. Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference. BMC Structural Biology. 2007, roč. 7, č. 36, s. 1-13. ISSN 1472-6807. |
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@article{718816,
author = {Adam, Jan and Pokorná, Martina and Sabin, Charles and Mitchell, Edward P. and Imberty, Anne and Wimmerová, Michaela},
article_number = {36},
keywords = {lectin; pseudomonas aeruginosa; protein engineering; microcalorimetry;X-ray},
language = {eng},
issn = {1472-6807},
journal = {BMC Structural Biology},
title = {Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference},
volume = {7},
year = {2007}
}
TY - JOUR
ID - 718816
AU - Adam, Jan - Pokorná, Martina - Sabin, Charles - Mitchell, Edward P. - Imberty, Anne - Wimmerová, Michaela
PY - 2007
TI - Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference
JF - BMC Structural Biology
VL - 7
IS - 36
SP - 1-13
EP - 1-13
SN - 14726807
KW - lectin
KW - pseudomonas aeruginosa
KW - protein engineering
KW - microcalorimetry;X-ray
N2 - Mutagenesis of amino acids forming the specificity binding loop in PA-IIL allowed identification of one amino acid that is crucial for definition of the lectin sugar preference. Altering specificity loop amino acids causes changes in saccharide-binding preferences of lectins derived from PA-IIL, via creation or blocking possible binding interactions. This finding opens a gate towards protein engineering and subsequent protein design to refine the desired binding properties and preferences, an approach that could have strong potential for drug design.
ER -
ADAM, Jan, Martina POKORNÁ, Charles SABIN, Edward P. MITCHELL, Anne IMBERTY a Michaela WIMMEROVÁ. Engineering of PA-IIL lectin from Pseudomonas aeruginosa - Unravelling the role of the specificity loop for sugar preference. \textit{BMC Structural Biology}. 2007, roč.~7, č.~36, s.~1-13. ISSN~1472-6807.
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