| RŮŽIČKA, Filip, Marie HORKÁ, Veronika HOLÁ a Miroslav VOTAVA. Capillary isoelectric focusing-useful tool for detection of the biofilm formation in Staphylococcus epidermidis. Journal of Microbiological Methods. Amsterdam: Elsevier Science B.V., 2006, roč. 68, č. 3, s. 530-535. ISSN 0167-7012. |
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@article{719266,
author = {Růžička, Filip and Horká, Marie and Holá, Veronika and Votava, Miroslav},
article_location = {Amsterdam},
article_number = {3},
keywords = {biofilm; Staphylococcus epidermidis; Capillary isoelectric focusing},
language = {eng},
issn = {0167-7012},
journal = {Journal of Microbiological Methods},
title = {Capillary isoelectric focusing-useful tool for detection of the biofilm formation in Staphylococcus epidermidis.},
volume = {68},
year = {2006}
}
TY - JOUR
ID - 719266
AU - Růžička, Filip - Horká, Marie - Holá, Veronika - Votava, Miroslav
PY - 2006
TI - Capillary isoelectric focusing-useful tool for detection of the biofilm formation in Staphylococcus epidermidis.
JF - Journal of Microbiological Methods
VL - 68
IS - 3
SP - 530-535
EP - 530-535
PB - Elsevier Science B.V.
SN - 01677012
KW - biofilm
KW - Staphylococcus epidermidis
KW - Capillary isoelectric focusing
N2 - The biofilm formation is an important factor of S. epidermidis virulence. Biofilm-positive strains might be clinically more important than biofilm-negative ones. Unlike biofilm-negative staphylococci, biofilm-positive staphylococci are surrounded with an extracellular polysaccharide substance. The presence of this substance on the surface can affect physico-chemical properties of the bacterial cell, including surface charge. 73 S. epidermidis strains were examined for the presence of ica operon, for the ability to form biofilm by Christensen test tube method and for the production of slime by Congo red agar method. Isoelectric points (pI) of these strains were determined by means of Capillary Isoelectric Focusing. The biofilm negative strains focused near pI value 2.3, while the pI values of the biofilm positive strains were near 2.6.
ER -
RŮŽIČKA, Filip, Marie HORKÁ, Veronika HOLÁ a Miroslav VOTAVA. Capillary isoelectric focusing-useful tool for detection of the biofilm formation in Staphylococcus epidermidis. \textit{Journal of Microbiological Methods}. Amsterdam: Elsevier Science B.V., 2006, roč.~68, č.~3, s.~530-535. ISSN~0167-7012.
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