Capillary isoelectric focusing and fluorometric detection of
proteins and microorganisms dynamically modified by
poly(ethylene glycol) pyrenebutanoate.

J 2006

Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.

HORKÁ, Marie, Filip RŮŽIČKA, Jaroslav HORKÝ, Veronika HOLÁ, Karel ŠLAIS et. al.

Basic information

Original name

Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.

Name in Czech

Kapilární izoelektrická fokusace a fluorimetrická detekce dynamicky modifikovaných proteinů a mikroorganizmů

Authors

HORKÁ, Marie (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, guarantor), Jaroslav HORKÝ (203 Czech Republic), Veronika HOLÁ (203 Czech Republic) and Karel ŠLAIS (203 Czech Republic)

Edition

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2006, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30300 3.3 Health sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.646

RIV identification code

RIV/00216224:14110/06:00016450

Organization unit

Faculty of Medicine

UT WoS

000242824900034

Keywords (in Czech)

kapilární izoelektrická fokusace, fluorometrická detekce; proteiny; mikroorganismy; poly(ethylen-glykol) máselná kyselina

Keywords in English

capillary isoelectric focusing; fluorometric detection; proteins; microorganisms; poly(ethylene glycol) pyrenebutanoate.

Abstract

ORIG CZ

V originále

The nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. This dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. The values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pI markers and were found comparable with pI of the native compounds. The mixed cultures of proteins and microorganisms, Escherichia coli CCM 3954, Staphylococcus epidermidis CCM 4418, Proteus vulgaris, Enterococcus faecalis CCM 4224, and Stenotrophomonas maltophilia, the strains of the yeast cells, Candida albicans CCM 8180, Candida krusei, Candida parapsilosis, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae were reproducibly focused and separated by the suggested technique. Using UV excitation for the on-column fluorometric detection, the minimum detectable amount was down to 10 cells injected on the separation capillary.

In Czech

Neionogenní tenzid poly(ethylen glycol) pyrenebutanoát byl použit při CIEF s fluorimetrickou detekcí. Toto barvivo dynamicky modifikovalo analyzované částice a umožnilo tak jejich citlivou detekci a identifikaci.

Links

IAA4031302, research and development project

Name: Využití rychlé elektroforetické separace s velmi citlivou fluorimetrickou detekcí pro identifikaci mikroorganismů

Investor: Academy of Sciences of the Czech Republic

Citovat

HORKÁ, Marie, Filip RŮŽIČKA, Jaroslav HORKÝ, Veronika HOLÁ and Karel ŠLAIS. Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2006, vol. 78, No 24, p. 8438-8444, 8 pp. ISSN 0003-2700.

@article{719272,
   author = {Horká, Marie and Růžička, Filip and Horký, Jaroslav and Holá, Veronika and Šlais, Karel},
   article_location = {Washington, D.C., USA},
   article_number = {24},
   keywords = {capillary isoelectric focusing; fluorometric detection; proteins; microorganisms; poly(ethylene glycol) pyrenebutanoate.},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.},
   volume = {78},
   year = {2006}
}

TY  - JOUR
ID  - 719272
AU  - Horká, Marie - Růžička, Filip - Horký, Jaroslav - Holá, Veronika - Šlais, Karel
PY  - 2006
TI  - Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate.
JF  - Analytical Chemistry
VL  - 78
IS  - 24
SP  - 8438-8444
EP  - 8438-8444
PB  - American Chemical Society
SN  - 00032700
KW  - capillary isoelectric focusing
KW  - fluorometric detection
KW  - proteins
KW  - microorganisms
KW  - poly(ethylene glycol) pyrenebutanoate.
N2  - The nonionogenic pyrene-based tenside, poly(ethylene glycol) pyrenebutanoate, was prepared and applied in capillary isoelectric focusing with fluorometric detection. This dye was used here as a buffer additive in capillary isoelectric focusing for a dynamic modification of the sample of proteins and microorganisms. The values of the isoelectric points of the labeled bioanalytes were calculated with use of the fluorescent pI markers and were found comparable with pI of the native compounds. The mixed cultures of proteins and microorganisms, Escherichia coli CCM 3954, Staphylococcus epidermidis CCM 4418, Proteus vulgaris, Enterococcus faecalis CCM 4224, and Stenotrophomonas maltophilia, the strains of the yeast cells, Candida albicans CCM 8180, Candida krusei, Candida parapsilosis, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae were reproducibly focused and separated by the suggested technique. Using UV excitation for the on-column fluorometric detection, the minimum detectable amount was down to 10 cells injected on the separation capillary.
ER  -

HORKÁ, Marie, Filip RŮŽIČKA, Jaroslav HORKÝ, Veronika HOLÁ and Karel ŠLAIS. Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2006, vol.~78, No~24, p.~8438-8444, 8 pp. ISSN~0003-2700.

Detailed Information on Publication Record