Antibodies against sulfur mustard and direct piezoelectric
immunosensing in an organic solvent

J 2007

Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent

SKLÁDAL, Petr, Jan PŘIBYL a Bohuslav ŠAFÁŘ

Základní údaje

Originální název

Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent

Název česky

Protilátky proti yperitu a přímý piezoelektrický imunosensor v organickém rozpouštědle

Autoři

SKLÁDAL, Petr (203 Česká republika, garant), Jan PŘIBYL (203 Česká republika) a Bohuslav ŠAFÁŘ (203 Česká republika)

Vydání

Analytical Letters, New York, Marcel Dekker, 2007, 0003-2719

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.362

Kód RIV

RIV/00216224:14310/07:00022212

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000246949900010

Klíčová slova anglicky

Sulfur mustard; antibody; immunoassay in organic phase; piezoelectric

Štítky

antibody, immunoassay in organic phase, piezoelectric, sulfur mustard

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 1. 2008 14:01, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

The development of polyclonal and monoclonal antibodies against sulfur mustard (SM; yperite) and initial experiments towards the piezoelectric immunosensor are reported. The antibodies were produced using bovine serum albumin and thyroglobulin as carrier proteins, the hapten density was 9-11 SM molecules per one molecule of albumin. The obtained antibodies were able to bind to the piezoelectric quartz crystal sensors with immobilized SM; however, the interaction with free SM was very weak in a competitive assay format. For this reason, the sensors with immobilized antibodies were prepared and tested for affinity to SM using toluene as solvent. Specific binding of SM to polyclonal antibodies was observed in dry toluene, the monoclonal antibody 2C1.2 was able to bind SM in toluene saturated with water. The preliminary results seem promising for immunosensing of SM in organic solvents.

Česky

Příprava protilátek proti yperitu a jejich testování v organickém rozpouštědle - toluenu - pomocí piezoelektrického imunosensoru.

Návaznosti

LC06030, projekt VaV

Název: Biomolekulární centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

SKLÁDAL, Petr, Jan PŘIBYL a Bohuslav ŠAFÁŘ. Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent. Analytical Letters. New York: Marcel Dekker, 2007, roč. 40, č. 7, s. 1347-1359. ISSN 0003-2719.

@article{719464,
   author = {Skládal, Petr and Přibyl, Jan and Šafář, Bohuslav},
   article_location = {New York},
   article_number = {7},
   keywords = {Sulfur mustard; antibody; immunoassay in organic phase; piezoelectric},
   language = {eng},
   issn = {0003-2719},
   journal = {Analytical Letters},
   note = {DOI: 10.1080/00032710701326700},
   title = {Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent},
   url = {http://www.informaworld.com/smpp/content~content=a779255771~db=all~order=page},
   volume = {40},
   year = {2007}
}

TY  - JOUR
ID  - 719464
AU  - Skládal, Petr - Přibyl, Jan - Šafář, Bohuslav
PY  - 2007
TI  - Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent
JF  - Analytical Letters
VL  - 40
IS  - 7
SP  - 1347-1359
EP  - 1347-1359
PB  - Marcel Dekker
SN  - 00032719
N1  - DOI: 10.1080/00032710701326700
KW  - Sulfur mustard
KW  - antibody
KW  - immunoassay in organic phase
KW  - piezoelectric
UR  - http://www.informaworld.com/smpp/content~content=a779255771~db=all~order=page
N2  - The development of polyclonal and monoclonal antibodies against sulfur mustard (SM; yperite) and initial experiments towards the piezoelectric immunosensor are reported. The antibodies were produced using bovine serum albumin and thyroglobulin as carrier proteins, the hapten density was 9-11 SM molecules per one molecule of albumin. The obtained antibodies were able to bind to the piezoelectric quartz crystal sensors with immobilized SM; however, the interaction with free SM was very weak in a competitive assay format. For this reason, the sensors with immobilized antibodies were prepared and tested for affinity to SM using toluene as solvent. Specific binding of SM to polyclonal antibodies was observed in dry toluene, the monoclonal antibody 2C1.2 was able to bind SM in toluene saturated with water. The preliminary results seem promising for immunosensing of SM in organic solvents.
ER  -

SKLÁDAL, Petr, Jan PŘIBYL a Bohuslav ŠAFÁŘ. Antibodies against sulfur mustard and direct piezoelectric immunosensing in an organic solvent. \textit{Analytical Letters}. New York: Marcel Dekker, 2007, roč.~40, č.~7, s.~1347-1359. ISSN~0003-2719.

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