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@inproceedings{720140,
author = {Juřica, Jan and Klepárník, Martin and Zahradníková, Lucia and Zendulka, Ondřej and Tomandl, Josef},
address = {Belgium},
booktitle = {HPLC 2007. Abstract Book.},
keywords = {HPLC; electrochemical detection; diclofenac; rat; cytochrome P450; CYP2C6},
language = {eng},
location = {Belgium},
pages = {723-723},
publisher = {I.O.P.M.S. vzw},
title = {HPLC - ED determination of diclofenac and its hydroxylated metabolite as a marker of metabolic activity of CYP2C6 in rats},
url = {http://www.hplc2007.org/},
year = {2007}
}
TY - JOUR
ID - 720140
AU - Juřica, Jan - Klepárník, Martin - Zahradníková, Lucia - Zendulka, Ondřej - Tomandl, Josef
PY - 2007
TI - HPLC - ED determination of diclofenac and its hydroxylated metabolite as a marker of metabolic activity of CYP2C6 in rats
PB - I.O.P.M.S. vzw
CY - Belgium
KW - HPLC
KW - electrochemical detection
KW - diclofenac
KW - rat
KW - cytochrome P450
KW - CYP2C6
UR - http://www.hplc2007.org/
N2 - Diclofenac belongs to the group of nonsteroidal antiinflammatory drugs. Presented method allows to determine concentration of diclofenac and its metabolite 4-hydroxydiclofenac in rat liver perfusate. The isolated rat liver was perfused with Williams medium E, the marker was administered as a bolus. The samples were collected in the 30th, 60th and 120th minute after the administration. Phenacetine was used as an internal standard (I.S.). After an addition of I.S. and pH adjustment, the analytes were extracted into diethyl ether and dried out under a gentle stream of nitrogen. The evaporated organic extract was dissolved in a mobile phase and analyzed on column Luna (2) C18 using coularray detection. The coulometric detection provides better sensitivity and limits of detection comparing to the conventional HPLC-UV methods. This method is suitable for modeling and description of possible pharmacological interactions on isoform CYP2C6.
ER -
JUŘICA, Jan, Martin KLEPÁRNÍK, Lucia ZAHRADNÍKOVÁ, Ondřej ZENDULKA a Josef TOMANDL. HPLC - ED determination of diclofenac and its hydroxylated metabolite as a marker of metabolic activity of CYP2C6 in rats. In \textit{HPLC 2007. Abstract Book.}. Belgium: I.O.P.M.S. vzw, 2007, s.~723-723.
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