2007
AhR-mediated and antiestrogenic activity of humic substances
JANOŠEK, Jaroslav; Michal BITTNER; Klára HILSCHEROVÁ; Luděk BLÁHA; J.P. GIESY et al.Basic information
Original name
AhR-mediated and antiestrogenic activity of humic substances
Name in Czech
AhR-zprostředkovaná a antiestrogenní aktivita huminových látek
Authors
Edition
Chemosphere, Oxford, UK, Elsevier Science Ltd. 2007, 0045-6535
Other information
Language
English
Type of outcome
Article in a journal
Field of Study
30304 Public and environmental health
Country of publisher
Czech Republic
Confidentiality degree
is not subject to a state or trade secret
Impact factor
Impact factor: 2.739
Marked to be transferred to RIV
Yes
RIV identification code
RIV/00216224:14310/07:00020344
Organization unit
Faculty of Science
UT WoS
Keywords in English
Ah receptor;AhR-mediated activity;Antiestrogenic effects;Endocrine disruption
Changed: 18/3/2010 10:16, prof. RNDr. Luděk Bláha, Ph.D.
In the original language
Humic substances (HS) were for decades regarded as inert in the ecosystems with respect to their possible toxicity. However, HS have been recently shown to elicit various adverse effects generally attributed to xenobiotics. In our study, we used MVLN and H4IIE-luc cell lines stably transfected with luciferase gene under control of estrogen receptor (ER) and Ah receptor (AhR; receptor connected with so-called dioxin-like toxicity) for assessment of anti/estrogenic and AhR-mediated effects of 12 commercially available humic substances. Out of those, five humic acids were shown to induce AhR-mediated activity with relative potencies related to TCDD 2.6 x 10-8-7.4 x 10-8. Organic extracts of HS solutions also elicited high activities what means that lipophilic molecules are responsible for a great part of effect. However, relatively high activity remaining in extracted solution suggests also presence of polar AhR-agonists. Contribution of persistent organic compounds to the observed effects was ruled out by H2SO4 treatment. Eight out of twelve HS elicited significant antiestrogenic effects with IC50 ranging from 40 to 164 mg l-1. The possible explanations of the antiestrogenic effect include sorption of 17-[beta]-estradiol (E2) on HS, changes in membrane permeability for E2 or another specific mechanism.
In Czech
doplnit
Links
| GP525/05/P160, research and development project |
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| MSM0021622412, plan (intention) |
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