INTERFERENCE OF PAHs AND THEIR DERIVATIVES WITH SIGNALING OF RETINOIDS

BENÍŠEK, Martin, Luděk BLÁHA, Jiří PACHERNÍK and Klára HILSCHEROVÁ. INTERFERENCE OF PAHs AND THEIR DERIVATIVES WITH SIGNALING OF RETINOIDS. In Abstracts of International Conference on Ecotoxicology - Trends and perspectives. 2006.

Basic information

• Original name: INTERFERENCE OF PAHs AND THEIR DERIVATIVES WITH SIGNALING OF RETINOIDS
• Name in Czech: Vliv polycyklikcýh aromatických uhlovodíků a jejich derivátů na retinoidní signálování
• Authors: BENÍŠEK, Martin (203 Czech Republic, belonging to the institution), Luděk BLÁHA (203 Czech Republic, belonging to the institution), Jiří PACHERNÍK (203 Czech Republic, belonging to the institution) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Abstracts of International Conference on Ecotoxicology - Trends and perspectives, 2006.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30304 Public and environmental health
• Country of publisher: Poland
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/06:00016464
• Organization unit: Faculty of Science
• Keywords in English: PAHs; retinoid; RAR/RXR; embryotoxicity; retinoic acid
• Tags: embryotoxicity, PaHs, RAR/RXR, retinoic acid, retinoid
• Tags: International impact

Abstract

For studying the disruption, we used new in vitro method with the cell line P19/A15. It is murine embryonal P19 cell line stably transfected with luciferase reporter gene under the control of RARE (Retinoic Acid Responsive Element). Xenobiotics potentially interacting with the retinoid signaling can either induce luciferase expression and/or negatively affect retinoic acid (ATRA)-induced luciferase activity. For our study we selected the group of PAH and their N-heterocyclic analogs especially for their widespread environmental occurrence and possible teratogenic effects. We tested 13 N-PAHs and 6 PAHs for retinoid and antiretinoid activity.

Abstract (in Czech)

For studying the disruption, we used new in vitro method with the cell line P19/A15. It is murine embryonal P19 cell line stably transfected with luciferase reporter gene under the control of RARE (Retinoic Acid Responsive Element). Xenobiotics potentially interacting with the retinoid signaling can either induce luciferase expression and/or negatively affect retinoic acid (ATRA)-induced luciferase activity. For our study we selected the group of PAH and their N-heterocyclic analogs especially for their widespread environmental occurrence and possible teratogenic effects. We tested 13 N-PAHs and 6 PAHs for retinoid and antiretinoid activity.

Links

• GP525/05/P160, research and development project: Name: In vitro modely pro studium endokrinní disrupce - účinky tradičních a nových typů persistentních organických polutantů

Investor: Czech Science Foundation, In vitro models for study of endocrine disruption - effects of traditional and new types of persistent organic pollutants

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)