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@article{720469,
author = {Bittner, Michal and Hilscherová, Klára and Giesy, John, Paul},
article_location = {Amsterdam, The Netherlands},
article_number = {33},
keywords = {Humic acids; Ah receptor; H4IIE-luc; Absorbance; NOM},
language = {eng},
issn = {0160-4120},
journal = {Environment International},
title = {Changes of AhR-mediated activity of humic substances after irradiation},
volume = {33},
year = {2007}
}
TY - JOUR
ID - 720469
AU - Bittner, Michal - Hilscherová, Klára - Giesy, John, Paul
PY - 2007
TI - Changes of AhR-mediated activity of humic substances after irradiation
JF - Environment International
VL - 33
IS - 33
SP - 812-816
EP - 812-816
PB - Elsevier
SN - 01604120
KW - Humic acids
KW - Ah receptor
KW - H4IIE-luc
KW - Absorbance
KW - NOM
N2 - Humic substances (HS) and natural organic matter (NOM) are natural organic compounds ubiquitous in the environment. However, some studies indicate that both HS and NOM can act as xenobiotics, e.g. induce hormone like effects in fish, amphibians and invertebrates. Molecules of these substances contain a number of aromatic rings and conjugated double bonds, the so called chromophores. Irradiation of dissolved HS and NOM can lead to a series of photochemical reactions which can act on these substances itself, or on other substances present in aquatic environment along with HS and NOM such as e.g. xenobiotics. In our previous study, we have found significant interactions of five humic acids (HA) with cytosolic aryl hydrocarbon receptor (AhR) in an in vitro bioassay based on H4IIEluc cells. In the present study, we have studied the changes in AhR mediated activities both of HS and NOM after irradiation that simulated natural solar light. Nine different HS and two NOM samples were irradiated in Pyrex tubes with a medium pressure mercury lamp for a duration of 0 to 52 h (which corresponds to 0 to 52 d natural solar radiation). Original concentrations of the samples were 50 mg L, and the greatest concentration of HS and NOM photoproducts subsequently tested in the bioassay was 17 mg L, which is an environmentally relevant concentration. After irradiation the absorbances of all the samples were less than the original materials. The AhR mediated activity of the HA Fluka and HA Sodium Salt were partially decreased by irradiation. The activities of other HS and NOM, that were either AhR active or inactive were not changed by irradiation. The results of the study demonstrate that AhRmediated activities of two active HA is caused by both photostable and photolabile AhR activators, while the other three active HA contain only photostable AhR activators. Potential mechanisms of the observed irradiation induced changes in AhRmediated activities are discussed.
ER -
BITTNER, Michal, Klára HILSCHEROVÁ and John, Paul GIESY. Changes of AhR-mediated activity of humic substances after irradiation. \textit{Environment International}. Amsterdam, The Netherlands: Elsevier, 2007, vol.~33, No~33, p.~812-816, 6 pp. ISSN~0160-4120.
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