Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection
| KLEPÁRNÍK, Martin and Josef TOMANDL. Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection. In Book of Abstracts. Olomouc: Palacký University Olomouc, 2007, p. 98-98. ISBN 978-80-244-1705-9. |
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| Basic information | |
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| Original name | Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection |
| Name in Czech | Stanovení dimethylovaných argininů v lidské plazmě pomocí HPLC s fluorescenční detekcí |
| Authors | KLEPÁRNÍK, Martin and Josef TOMANDL. |
| Edition | Olomouc, Book of Abstracts, p. 98-98, 1 pp. 2007. |
| Publisher | Palacký University Olomouc |
| Other information | |
|---|---|
| Original language | English |
| Type of outcome | Proceedings paper |
| Field of Study | 10406 Analytical chemistry |
| Country of publisher | Czech Republic |
| Confidentiality degree | is not subject to a state or trade secret |
| WWW | Advances in chromatography and electrophoresis 2007 & Chiranal 2007 |
| Organization unit | Faculty of Medicine |
| ISBN | 978-80-244-1705-9 |
| Keywords in English | assymetric dimethylarginine; HPLC |
| Tags | assymetric dimethylarginine, HPLC |
| Tags | International impact |
| Changed by | Changed by: doc. RNDr. Josef Tomandl, Ph.D., učo 47. Changed: 28/6/2007 11:40. |
| Abstract |
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| Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. We report here development of a new HPLC method for the determination of ADMA using fluorescent detection. After solid-phase extraction procedure and evaporation of the eluate under gentle stream of nitrogen the reconstituted samples were derivatised with an ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a Hydro-RP column using isocratic elution and fluorescence detection. |
| Abstract (in Czech) |
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| Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. We report here development of a new HPLC method for the determination of ADMA using fluorescent detection. After solid-phase extraction procedure and evaporation of the eluate under gentle stream of nitrogen the reconstituted samples were derivatised with an ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a Hydro-RP column using isocratic elution and fluorescence detection. |
| Links | |
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| GA203/06/0047, research and development project | Name: Využití kapilární elektroforézy pro studium metabolismu léčiv |
| Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies | |
| LC06023, research and development project | Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie |
| Investor: Ministry of Education, Youth and Sports of the CR | |
| MSM0021622402, plan (intention) | Name: Časná diagnostika a léčba kardiovaskulárních chorob |
| Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases | |
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