Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection
KLEPÁRNÍK, Martin and Josef TOMANDL. Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection. In Book of Abstracts. Olomouc: Palacký University Olomouc, 2007, p. 98-98. ISBN 978-80-244-1705-9. |
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Basic information | |
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Original name | Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection |
Name in Czech | Stanovení dimethylovaných argininů v lidské plazmě pomocí HPLC s fluorescenční detekcí |
Authors | KLEPÁRNÍK, Martin and Josef TOMANDL. |
Edition | Olomouc, Book of Abstracts, p. 98-98, 1 pp. 2007. |
Publisher | Palacký University Olomouc |
Other information | |
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Original language | English |
Type of outcome | Proceedings paper |
Field of Study | 10406 Analytical chemistry |
Country of publisher | Czech Republic |
Confidentiality degree | is not subject to a state or trade secret |
WWW | Advances in chromatography and electrophoresis 2007 & Chiranal 2007 |
Organization unit | Faculty of Medicine |
ISBN | 978-80-244-1705-9 |
Keywords in English | assymetric dimethylarginine; HPLC |
Tags | assymetric dimethylarginine, HPLC |
Tags | International impact |
Changed by | Changed by: doc. RNDr. Josef Tomandl, Ph.D., učo 47. Changed: 28/6/2007 11:40. |
Abstract |
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Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. We report here development of a new HPLC method for the determination of ADMA using fluorescent detection. After solid-phase extraction procedure and evaporation of the eluate under gentle stream of nitrogen the reconstituted samples were derivatised with an ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a Hydro-RP column using isocratic elution and fluorescence detection. |
Abstract (in Czech) |
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Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. We report here development of a new HPLC method for the determination of ADMA using fluorescent detection. After solid-phase extraction procedure and evaporation of the eluate under gentle stream of nitrogen the reconstituted samples were derivatised with an ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a Hydro-RP column using isocratic elution and fluorescence detection. |
Links | |
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GA203/06/0047, research and development project | Name: Využití kapilární elektroforézy pro studium metabolismu léčiv |
Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies | |
LC06023, research and development project | Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie |
Investor: Ministry of Education, Youth and Sports of the CR | |
MSM0021622402, plan (intention) | Name: Časná diagnostika a léčba kardiovaskulárních chorob |
Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases |
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