2007
Cytochrome P450 2C9 Activity Study by Micellar Electrokinetic Capillary Chromatography (Lecture)
KONEČNÝ, Jiří a Zdeněk GLATZZákladní údaje
Originální název
Cytochrome P450 2C9 Activity Study by Micellar Electrokinetic Capillary Chromatography (Lecture)
Název česky
Stanovení aktivity cytochromu P450 2C9 pomocí MEKC (Přednáška)
Autoři
KONEČNÝ, Jiří a Zdeněk GLATZ
Vydání
Olomouc, Book of Abstracts, od s. 98-98, 1 s. 2007
Nakladatel
Palacký University Olomouc
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-80-244-1705-9
Klíčová slova anglicky
assymetric dimethylarginine; HPLC
Štítky
Příznaky
Mezinárodní význam
Změněno: 19. 5. 2009 18:44, prof. RNDr. Zdeněk Glatz, CSc.
V originále
Cytochromes P45O are involved in a wide variety of metabolic and biosynthetic processes encompassing drug metabolism. Cytochrome P450 2C9 (CYP2C9) is one of the most important isoform in human liver involved in the metabolism of a large number of therapeutic agents. Predominantly high performance liquid chromatography (HPLC) with UV and/or fluorescence detection is chosen for these studies. By reason of sample saving and minor time demands, the possibility of automation, CE analysis is suitable for studies comprising work with enzymes, substrates as well as inhibitors. The applicability of capillary electrophoresis (CE) for the determination of enzymatic activity of CYP2C9 with diclofenac as a probe substrate is demonstrated. Micellar electrokinetic capillary chromatography (MEKC) with SDS as a pseudostationary phase was used for this purpose. The kinetic study on the given enzymatic reaction was also performed since basic characterization of drug biotransformation generally begins with the enzyme kinetic analysis of metabolite formation. As a result Michaelis constant and maximum reaction velocity were evaluated, the values 3.44 uM respectively 19.78 nmol.min-1.nmol-1 were in agreement with the literature data. Moreover, the proposed method is suitable for assessing of substrate inhibition study using sulfaphenazole as an inhibitor of CYP2C9 activity. As a result the IC50 value for sulfaphenazole was determinated 0.29 uM which is in consistence with those reported in literature.
Česky
Byla vypracována metoda stanovení aktivity cytochromu P450 2C9 pomocí MEKC.
Návaznosti
| GA203/06/0047, projekt VaV |
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| LC06023, projekt VaV |
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| MSM0021622413, záměr |
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