Detailed Information on Publication Record
2007
Is GC-content Correlated with Genome Size in Plants
BUREŠ, Petr, Petr ŠMARDA, Ivana HRALOVÁ, Klára HELÁNOVÁ, Sara FUENTES-SORIANO et. al.Basic information
Original name
Is GC-content Correlated with Genome Size in Plants
Name in Czech
Koreluje obsah guaninu a cytosinu s velikostí genomu u rostlin?
Authors
BUREŠ, Petr (203 Czech Republic, guarantor, belonging to the institution), Petr ŠMARDA (203 Czech Republic, belonging to the institution), Ivana HRALOVÁ (203 Czech Republic), Klára HELÁNOVÁ (203 Czech Republic, belonging to the institution), Sara FUENTES-SORIANO (484 Mexico) and Jana PROCHÁZKOVÁ (203 Czech Republic)
Edition
Plant Genome Horizons - Vistas & Visions, 2007
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/07:00022337
Organization unit
Faculty of Science
Keywords in English
GC-content; Angiosperms; base composition; c-value; Apiaceae; Brassicaceae; Carex; Eleocharis; Festuca; Epilobium; Festuca
Tags
Tags
International impact
Změněno: 26/3/2019 21:59, prof. RNDr. Petr Bureš, Ph.D.
V originále
GC base pair content is an important character in the description of new taxa of prokaryotes, but its role in plant systematics and evolution is still poorly understood. Although GC content is positively correlated with genome size in bacteria and vertebrates (1) there is debate about how those two factors are related in plants. Barow and Meister (2) estimated base composition and genome size in 54 taxa of angiosperms and gymnosperms, and their findings did not support Vinogradov (1) proposition stating that there is a positive correlation between GC content and genome size. More recently, Barow and Meister (3) confirmed that base composition and genome size were not correlated using an expanded sampling of 215 plant species measured by numerous authors over the last 14 years. Using flow cytometry methods (FCM) with PI and DAPI, we estimated base composition in closely related species of Apiaceae (16 spp.), Brassicaceae (30 spp.), Cyperaceae (135 spp.), Onagraceae (15 spp.), Poaceae (132 spp.) and Salicaceae (20 spp.), and found a significant positive correlation between GC content and either genome size or monoploid genome size. Sequence data of 11 Oryza species (4) independently supported our FCM results and suggested that GC and genome size correlation is explained by genomic repeats and interspecific genome size variation found within closely related taxa. 1. Vinogradov AE (1994): Cytometry 16: 34-40. 2. Barow M & Meister A (2002): Cytometry 47: 1-7. 3. Meister A & Barow M (2007): Analysis of Base Composition by Flow Cytometry. In: Doležel J et al. (eds.), Flow Cytometry with Plant Cells. Wiley, Weinheim, pp. 177-215. 4. Ammiraju JSS et al. (2006): Genome Research 16: 140-147.
In Czech
GC base pair content is an important character in the description of new taxa of prokaryotes, but its role in plant systematics and evolution is still poorly understood. Although GC content is positively correlated with genome size in bacteria and vertebrates (1) there is debate about how those two factors are related in plants. Barow and Meister (2) estimated base composition and genome size in 54 taxa of angiosperms and gymnosperms, and their findings did not support Vinogradov (1) proposition stating that there is a positive correlation between GC content and genome size. More recently, Barow and Meister (3) confirmed that base composition and genome size were not correlated using an expanded sampling of 215 plant species measured by numerous authors over the last 14 years. Using flow cytometry methods (FCM) with PI and DAPI, we estimated base composition in closely related species of Apiaceae (16 spp.), Brassicaceae (30 spp.), Cyperaceae (135 spp.), Onagraceae (15 spp.), Poaceae (132 spp.) and Salicaceae (20 spp.), and found a significant positive correlation between GC content and either genome size or monoploid genome size. Sequence data of 11 Oryza species (4) independently supported our FCM results and suggested that GC and genome size correlation is explained by genomic repeats and interspecific genome size variation found within closely related taxa. 1. Vinogradov AE (1994): Cytometry 16: 34-40. 2. Barow M & Meister A (2002): Cytometry 47: 1-7. 3. Meister A & Barow M (2007): Analysis of Base Composition by Flow Cytometry. In: Doležel J et al. (eds.), Flow Cytometry with Plant Cells. Wiley, Weinheim, pp. 177-215. 4. Ammiraju JSS et al. (2006): Genome Research 16: 140-147.
Links
| LC06073, research and development project |
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| MSM0021622416, plan (intention) |
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