TALIAN, Ivan, Andrej ORIŇÁK, Jan PREISLER, Andreas HEILE, Lucia ONOFREJOVÁ, Dušan KANIANSKÝ and Heinrich ARLINGHAUS. Comparative TOF-SIMS and MALDI TOF-MS analysis on different chromatographic planar substrates. Journal of Separation Science. Weinheim: WILEY-VCH Verlag GmbH & Co., 2007, vol. 30, No 16, p. 2570 - 2582. ISSN 1615-9306.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Comparative TOF-SIMS and MALDI TOF-MS analysis on different chromatographic planar substrates
Name in Czech TOF-SIMS a MALDI TOF-MS analýza na různých chromatografických tenkých vrstvách
Authors TALIAN, Ivan (703 Slovakia), Andrej ORIŇÁK (703 Slovakia), Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution), Andreas HEILE (276 Germany), Lucia ONOFREJOVÁ (203 Czech Republic, belonging to the institution), Dušan KANIANSKÝ (703 Slovakia) and Heinrich ARLINGHAUS (276 Germany).
Edition Journal of Separation Science, Weinheim, WILEY-VCH Verlag GmbH & Co. 2007, 1615-9306.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.632
RIV identification code RIV/00216224:14310/07:00022372
Organization unit Faculty of Science
UT WoS 000250986700002
Keywords in English TLC-MS; TOF-SIMS; MALDI TOF-MS; Fragmentation; Chromatographic substrates
Tags Chromatographic substrates, Fragmentation, MALDI TOF-MS, TLC-MS, TOF-SIMS
Tags International impact, Reviewed
Changed by Changed by: prof. Mgr. Jan Preisler, Ph.D., učo 45329. Changed: 31/7/2017 15:27.
Abstract
A comparison is made between two high resolution, surface-based, mass spectrometric methods: time-of-flight secondary ion mass spectrometry (TOF-SIMS) and matrix-assisted laser desorption/ionisation mass spectrometry (MALDI TOF-MS) in indication of abietic and gibberellic acids molecular profiles on different chromatographic thin layers. The analytes were applied to silica gel chromatographic thin layers with SIMS on-line interfacing channel, monolithic silica gel ultra-thin layers, and thin layers specifically designed for direct Raman spectroscopic analysis. Two MALDI matrices were used in this research: ferulic acid and 2,5-dihydroxybenzoic acid. The silica gel SIMS-interfacing channel strongly supported formation of numerous different MALDI MS fragments with abietic and gibberellic acids, and ferulic acid matrix. The most intense fragments belonged to [M-OH]+ and [M]+ ions from ferulic acid. Intense conjugates were detected with gibberellic acid. The MALDI MS spectrum from the monolithic silica gel surface showed very low analyte signal intensity and it was not possible to obtain MALDI spectra from a Raman spectroscopy treated chromatographic layer. The MALDI TOF MS gibberellic acid fragmentation profile was shielded by the matrix used and was accompanied by poor analyte identification. The most useful TOF-SIMS analytical signal response was obtained from analytes separated on monolithic silica gel and a SIMS-interfacing modified silica gel surface. New horizons with nanostructured surfaces call for high resolution MS methods (which cannot readily be miniaturised like many optical and electrochemical methods) to be integrated in chip and nanoscale detection systems.
Abstract (in Czech)
Srovnání TOF-SIMS a MALDI TOF-MS profilů biologicky významných organických kyselin na různých chromatografických tenkých vrstvách.
Links
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
PrintDisplayed: 21/5/2024 06:01