| KAŇUCH, Peter, Petra HÁJKOVÁ, Zdeněk ŘEHÁK a Josef BRYJA. A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples. Acta Chiropterologica. Warszawa: Museum and Institute of Zoology PAS, 2007, roč. 9, č. 1, s. 277-282. ISSN 1508-1109. |
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@article{721316,
author = {Kaňuch, Peter and Hájková, Petra and Řehák, Zdeněk and Bryja, Josef},
article_location = {Warszawa},
article_number = {1},
keywords = {Chiroptera; cytochrome b; mtDNA; sibling species; non-invasive sampling},
language = {eng},
issn = {1508-1109},
journal = {Acta Chiropterologica},
title = {A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples},
volume = {9},
year = {2007}
}
TY - JOUR
ID - 721316
AU - Kaňuch, Peter - Hájková, Petra - Řehák, Zdeněk - Bryja, Josef
PY - 2007
TI - A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples
JF - Acta Chiropterologica
VL - 9
IS - 1
SP - 277-282
EP - 277-282
PB - Museum and Institute of Zoology PAS
SN - 15081109
KW - Chiroptera
KW - cytochrome b
KW - mtDNA
KW - sibling species
KW - non-invasive sampling
N2 - The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology.
ER -
KAŇUCH, Peter, Petra HÁJKOVÁ, Zdeněk ŘEHÁK a Josef BRYJA. A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples. \textit{Acta Chiropterologica}. Warszawa: Museum and Institute of Zoology PAS, 2007, roč.~9, č.~1, s.~277-282. ISSN~1508-1109.
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