KAŇUCH, Peter, Petra HÁJKOVÁ, Zdeněk ŘEHÁK a Josef BRYJA. A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples. Acta Chiropterologica. Warszawa: Museum and Institute of Zoology PAS, 2007, roč. 9, č. 1, s. 277-282. ISSN 1508-1109. |
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@article{721316, author = {Kaňuch, Peter and Hájková, Petra and Řehák, Zdeněk and Bryja, Josef}, article_location = {Warszawa}, article_number = {1}, keywords = {Chiroptera; cytochrome b; mtDNA; sibling species; non-invasive sampling}, language = {eng}, issn = {1508-1109}, journal = {Acta Chiropterologica}, title = {A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples}, volume = {9}, year = {2007} }
TY - JOUR ID - 721316 AU - Kaňuch, Peter - Hájková, Petra - Řehák, Zdeněk - Bryja, Josef PY - 2007 TI - A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples JF - Acta Chiropterologica VL - 9 IS - 1 SP - 277-282 EP - 277-282 PB - Museum and Institute of Zoology PAS SN - 15081109 KW - Chiroptera KW - cytochrome b KW - mtDNA KW - sibling species KW - non-invasive sampling N2 - The identification of two cryptic bat species of the genus Pipistrellus using a non-destructive and quick method of multiplex PCR and agarose gel electrophoresis is described. Two primer combinations were able to produce species-specific bands that identified reliably individuals that were previously identified by mtDNA sequencing. Robustness of the method was subsequently successfully tested on 16 randomly selected free-living animals from central Europe (tissue samples obtained from a 3 mm punch of wing-membrane) identified to species on the basis of echolocation calls. Nine out of 15 museum specimens and 100% of fresh faecal samples from seven individuals were also successfully identified by this method. The described method thus provides a good way to routinely distinguish two Pipistrellus species by using non-destructive sampling of living individuals or droppings, and will be used in field studies of their ecology. ER -
KAŇUCH, Peter, Petra HÁJKOVÁ, Zdeněk ŘEHÁK a Josef BRYJA. A rapid PCR-based test for species identification of two cryptic bats Pipistrellus pipistrellus and P. pygmaeus and its application on museum and dropping samples. \textit{Acta Chiropterologica}. Warszawa: Museum and Institute of Zoology PAS, 2007, roč.~9, č.~1, s.~277-282. ISSN~1508-1109.
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