Large-scale Microarray Analysis of Protein and mRNA Level Changes in HL-60 Cells

ŠIMARA, Pavel, Irena KOUTNÁ, Stanislav STEJSKAL, Martina PETERKOVÁ, Petr KRONTORÁD and Zdeněk RUČKA. Large-scale Microarray Analysis of Protein and mRNA Level Changes in HL-60 Cells. 2007.

Basic information

• Original name: Large-scale Microarray Analysis of Protein and mRNA Level Changes in HL-60 Cells
• Name in Czech: Genomická a proteomická analýza buněk HL-60 pomocí microarray technologie.
• Authors: ŠIMARA, Pavel (203 Czech Republic, guarantor, belonging to the institution), Irena KOUTNÁ (203 Czech Republic, belonging to the institution), Stanislav STEJSKAL (203 Czech Republic, belonging to the institution), Martina PETERKOVÁ (203 Czech Republic, belonging to the institution), Petr KRONTORÁD (203 Czech Republic, belonging to the institution) and Zdeněk RUČKA (203 Czech Republic).
• Edition: 2007.

Other information

• Original language: English
• Type of outcome: Audiovisual works
• Field of Study: 10601 Cell biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14330/07:00022540
• Organization unit: Faculty of Informatics
• Keywords in English: HL-60; microarrays
• Tags: HL-60, microarrays
• Tags: International impact

Abstract

In this study we compare methods for large-scale microarray analysis of protein and RNA level changes in HL-60 cells, responding to differentiation stimuli. Using microarrays we have found, that level of several proteins was either up- or down-regulated after cell differentiation. In some cases there was significant correlation with appropriate genes.

Abstract (in Czech)

V této studii porovnáváme genomický a proteomický profil buněk HL-60.

Links

• MSM0021622430, plan (intention): Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies

• 2B06052, research and development project: Name: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)

Investor: Ministry of Education, Youth and Sports of the CR, Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images