2007
Genome rearrangements in host-range mutants of the plyvalent staphylococcal bacteriophage 812
KAŠPÁREK, Petr, Roman PANTŮČEK, Jana KAHÁNKOVÁ, Vladislava RŮŽIČKOVÁ, Jiří DOŠKAŘ et. al.Základní údaje
Originální název
Genome rearrangements in host-range mutants of the plyvalent staphylococcal bacteriophage 812
Název česky
Genomické změny u mutant polyvalentního fága 812 v rozmezí hostitele
Autoři
KAŠPÁREK, Petr (203 Česká republika), Roman PANTŮČEK (203 Česká republika), Jana KAHÁNKOVÁ (203 Česká republika), Vladislava RŮŽIČKOVÁ (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika, garant)
Vydání
Folia Microbiologica, Praha, Czechoslovak Society for Microbiology, 2007, 0015-5632
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 0.989
Kód RIV
RIV/00216224:14310/07:00020477
Organizační jednotka
Přírodovědecká fakulta
UT WoS
000250290500003
Klíčová slova anglicky
Staphylococcus bacteriophage; host-range; bacteriophage therapy; introns
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 1. 7. 2009 18:41, prof. RNDr. Roman Pantůček, Ph.D.
V originále
Mutations extended the host range of the polyvalent bacteriophage 812 of the family Myoviridae in up to 95 % of Staphylococcus aureus strains and 43 % of strains of different coagulase-positive and coagulase-negative Staphylococcus species. Mutational changes in the genome of several previously isolated host-range mutants of phage 812 were identified and described. Host-range mutant 812F1 harbors a deletion in endolysin gene that arose together with intron excision. Mutants 812i, 812b, 812p and 812F3 harbor deletion in the structural gene orf8 that results from a genome rearrangement that is associated with intron insertion. This rearrangement was also detected in the genome of the closely related phages, U16 and phi131. Another intron was discovered in the recA812 gene in mutants 812i, 812b, 812p and 812F3. An insertion was found in a non-coding region of the restriction fragment PstI-O of mutants 812b, 812F3 and 812g and phages U16 and phi131. Results of this study contribute to the explanation of genetic factors affecting the host range of polyvalent staphylococcal bacteriophages.
Česky
Mutations extended the host range of the polyvalent bacteriophage 812 of the family Myoviridae in up to 95 % of Staphylococcus aureus strains and 43 % of strains of different coagulase-positive and coagulase-negative Staphylococcus species. Mutational changes in the genome of several previously isolated host-range mutants of phage 812 were identified and described. Host-range mutant 812F1 harbors a deletion in endolysin gene that arose together with intron excision. Mutants 812i, 812b, 812p and 812F3 harbor deletion in the structural gene orf8 that results from a genome rearrangement that is associated with intron insertion. This rearrangement was also detected in the genome of the closely related phages, U16 and phi131. Another intron was discovered in the recA812 gene in mutants 812i, 812b, 812p and 812F3. An insertion was found in a non-coding region of the restriction fragment PstI-O of mutants 812b, 812F3 and 812g and phages U16 and phi131. Results of this study contribute to the explanation of genetic factors affecting the host range of polyvalent staphylococcal bacteriophages.
Návaznosti
| GA203/03/0515, projekt VaV |
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| MSM0021622415, záměr |
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