HORÁK, Daniel, Bohuslav RITTICH a Alena ŠPANOVÁ. Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products. Journal of Magnetism and Magnetic Materials. Amsterdam, Netherlands: Elsevier Science, 2007, roč. 311, č. 1, s. 249-254. ISSN 0304-8853. |
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@article{725491, author = {Horák, Daniel and Rittich, Bohuslav and Španová, Alena}, article_location = {Amsterdam, Netherlands}, article_number = {1}, keywords = {magnetic particles; DNA; magnetite; PCR; dispersion polymerization; bacterial DNA assay}, language = {eng}, issn = {0304-8853}, journal = {Journal of Magnetism and Magnetic Materials}, title = {Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products}, volume = {311}, year = {2007} }
TY - JOUR ID - 725491 AU - Horák, Daniel - Rittich, Bohuslav - Španová, Alena PY - 2007 TI - Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products JF - Journal of Magnetism and Magnetic Materials VL - 311 IS - 1 SP - 249-254 EP - 249-254 PB - Elsevier Science SN - 03048853 KW - magnetic particles KW - DNA KW - magnetite KW - PCR KW - dispersion polymerization KW - bacterial DNA assay N2 - Magnetite nanoparticles ca. 14 nm in diameter were obtained by chemical coprecipitation of Fe(II) and Fe(III) salts with ammonia in the presence of poly(ethylene glycol) (PEG). Magnetic poly(glycidyl methacrylate) (PGMA) microspheres were prepared by dispersion polymerization in poly(vinylpyrrolidone) (PVP)-stabilized aqueous ethanol with PEG-coated magnetite nanoparticles. Magnetic PGMA microspheres were hydrolyzed and carboxyl groups introduced by oxidation with KMnO4. Such a carrier was used for isolation of DNA. The particles reversibly bound DNA in the presence of high concentration of PEG 6,000 and sodium chloride. Bacterial DNA was isolated from crude cell lysates of different dairy products (butter milk, cheese, yoghurt), probiotic tablets or from cell lyophilisates. The presence of Bifidobacterium and Lactobacillus DNA in samples was identified by PCR amplification. ER -
HORÁK, Daniel, Bohuslav RITTICH a Alena ŠPANOVÁ. Carboxyl-functionalized magnetic microparticle carrier for isolation and identification of DNA in dairy products. \textit{Journal of Magnetism and Magnetic Materials}. Amsterdam, Netherlands: Elsevier Science, 2007, roč.~311, č.~1, s.~249-254. ISSN~0304-8853.
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