| RITTICH, Bohuslav, Alena ŠPANOVÁ, Petra NĚMCOVÁ a Daniel HORÁK. Solid-phase reversed immobilization of bacterial DNA from aqueous two phase system. In Ninth International Symposium on Advances in Extraction Technologies. Aalesund, Norway: University of Oslo, 2007, s. 208-208. |
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@inproceedings{725498,
author = {Rittich, Bohuslav and Španová, Alena and Němcová, Petra and Horák, Daniel},
address = {Aalesund, Norway},
booktitle = {Ninth International Symposium on Advances in Extraction Technologies},
keywords = {DNA; isolation; magnetic microspheres; P(HEMA-co-EDMA); aqueous two-phase system; PEG 6000; NaCl},
language = {eng},
location = {Aalesund, Norway},
pages = {208-208},
publisher = {University of Oslo},
title = {Solid-phase reversed immobilization of bacterial DNA from aqueous two phase system},
year = {2007}
}
TY - JOUR
ID - 725498
AU - Rittich, Bohuslav - Španová, Alena - Němcová, Petra - Horák, Daniel
PY - 2007
TI - Solid-phase reversed immobilization of bacterial DNA from aqueous two phase system
PB - University of Oslo
CY - Aalesund, Norway
KW - DNA
KW - isolation
KW - magnetic microspheres
KW - P(HEMA-co-EDMA)
KW - aqueous two-phase system
KW - PEG 6000
KW - NaCl
N2 - The aim of work was the isolation of PCR-ready DNA from dairy products by magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethylmethacrylate) (P(HEMA-co-EDMA)) microspheres in the presence of an aqueous two-phase system (composed of PEG 6000 and NaCl). DNA was apparently adsorbed on magnetic particles surface from two-phase aqueous (PEG 6000/NaCl) system. The sensitivity of PCR of the DNA isolation method tested was comparable with the phenol extraction method.
ER -
RITTICH, Bohuslav, Alena ŠPANOVÁ, Petra NĚMCOVÁ a Daniel HORÁK. Solid-phase reversed immobilization of bacterial DNA from aqueous two phase system. In \textit{Ninth International Symposium on Advances in Extraction Technologies}. Aalesund, Norway: University of Oslo, 2007, s.~208-208.
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