J 2007

Experimental design in intraspecific organelle DNA sequence studies III: statistical measures of sampling success

HOLT, Jason A, Sierra Dawn STONEBERG HOLT a Petr BUREŠ

Základní údaje

Originální název

Experimental design in intraspecific organelle DNA sequence studies III: statistical measures of sampling success

Název česky

Experimental design in intraspecific organelle DNA sequence studies III: statistical measures of sampling success

Autoři

HOLT, Jason A (840 Spojené státy), Sierra Dawn STONEBERG HOLT (840 Spojené státy) a Petr BUREŠ (203 Česká republika, garant, domácí)

Vydání

Taxon, Vienna, International Assoc. for Plant Taxonomy, 2007, 0040-0262

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.524

Kód RIV

RIV/00216224:14310/07:00022667

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000249247400020

Klíčová slova anglicky

bulking; haplotype frequency confidence intervals; inclusion-exclusion principle; intraspecific sequence studies; phylogeography; proportion estimates

Příznaky

Mezinárodní význam, Recenzováno
Změněno: 21. 4. 2011 12:47, prof. RNDr. Petr Bureš, Ph.D.

Anotace

V originále

Statistical methods are proposed for analyzing the experimental design, preliminary results, and final results of phylogenetic studies of organelle DNA sequence at low taxonomic levels. Such studies require sampling numerous individuals, many of which share identical haplotypes. The proportions of the haplotypes sampled can help answer the following questions: (1) Is one haplotype so dominant that the particular DNA region is without meaningful variation within the scope of the study? (2) Were all prevalent haplotypes found? (3) What are the proportions of each haplotype within the studied group? (4) What percentage of the studied group can be confidently asserted to belong to the haplotypes that were found? Examples are given in which the statistics techniques are applied to data drawn from the botanical literature. Tables are included as a quick reference for the researcher who wishes to circumvent calculation. A Microsoft Excel 2000 spreadsheet (titled HaploPro.xls) for performing some of the more complicated calculations is offered online. Finally, the limitations of these methods and their applicability to nuclear DNA and other characters studies are discussed.

Česky

Statistical methods are proposed for analyzing the experimental design, preliminary results, and final results of phylogenetic studies of organelle DNA sequence at low taxonomic levels. Such studies require sampling numerous individuals, many of which share identical haplotypes. The proportions of the haplotypes sampled can help answer the following questions: (1) Is one haplotype so dominant that the particular DNA region is without meaningful variation within the scope of the study? (2) Were all prevalent haplotypes found? (3) What are the proportions of each haplotype within the studied group? (4) What percentage of the studied group can be confidently asserted to belong to the haplotypes that were found? Examples are given in which the statistics techniques are applied to data drawn from the botanical literature. Tables are included as a quick reference for the researcher who wishes to circumvent calculation. A Microsoft Excel 2000 spreadsheet (titled HaploPro.xls) for performing some of the more complicated calculations is offered online. Finally, the limitations of these methods and their applicability to nuclear DNA and other characters studies are discussed.

Návaznosti

LC06073, projekt VaV
Název: Centrum pro výzkum biodiverzity
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum biodiverzity
MSM0021622416, záměr
Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase