HOLT, Jason A, Sierra Dawn STONEBERG HOLT a Petr BUREŠ. Experimental design in intraspecific organelle DNA sequence studies III: statistical measures of sampling success. Taxon. Vienna: International Assoc. for Plant Taxonomy, 2007, roč. 56/2007, č. 3, s. 847-856. ISSN 0040-0262.
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Základní údaje
Originální název Experimental design in intraspecific organelle DNA sequence studies III: statistical measures of sampling success
Název česky Experimental design in intraspecific organelle DNA sequence studies III: statistical measures of sampling success
Autoři HOLT, Jason A (840 Spojené státy), Sierra Dawn STONEBERG HOLT (840 Spojené státy) a Petr BUREŠ (203 Česká republika, garant, domácí).
Vydání Taxon, Vienna, International Assoc. for Plant Taxonomy, 2007, 0040-0262.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 10600 1.6 Biological sciences
Stát vydavatele Rakousko
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 2.524
Kód RIV RIV/00216224:14310/07:00022667
Organizační jednotka Přírodovědecká fakulta
UT WoS 000249247400020
Klíčová slova anglicky bulking; haplotype frequency confidence intervals; inclusion-exclusion principle; intraspecific sequence studies; phylogeography; proportion estimates
Štítky bulking, haplotype frequency confidence intervals, inclusion-exclusion principle, intraspecific sequence studies, phylogeography, proportion estimates
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnil: prof. RNDr. Petr Bureš, Ph.D., učo 2635. Změněno: 21. 4. 2011 12:47.
Anotace
Statistical methods are proposed for analyzing the experimental design, preliminary results, and final results of phylogenetic studies of organelle DNA sequence at low taxonomic levels. Such studies require sampling numerous individuals, many of which share identical haplotypes. The proportions of the haplotypes sampled can help answer the following questions: (1) Is one haplotype so dominant that the particular DNA region is without meaningful variation within the scope of the study? (2) Were all prevalent haplotypes found? (3) What are the proportions of each haplotype within the studied group? (4) What percentage of the studied group can be confidently asserted to belong to the haplotypes that were found? Examples are given in which the statistics techniques are applied to data drawn from the botanical literature. Tables are included as a quick reference for the researcher who wishes to circumvent calculation. A Microsoft Excel 2000 spreadsheet (titled HaploPro.xls) for performing some of the more complicated calculations is offered online. Finally, the limitations of these methods and their applicability to nuclear DNA and other characters studies are discussed.
Anotace česky
Statistical methods are proposed for analyzing the experimental design, preliminary results, and final results of phylogenetic studies of organelle DNA sequence at low taxonomic levels. Such studies require sampling numerous individuals, many of which share identical haplotypes. The proportions of the haplotypes sampled can help answer the following questions: (1) Is one haplotype so dominant that the particular DNA region is without meaningful variation within the scope of the study? (2) Were all prevalent haplotypes found? (3) What are the proportions of each haplotype within the studied group? (4) What percentage of the studied group can be confidently asserted to belong to the haplotypes that were found? Examples are given in which the statistics techniques are applied to data drawn from the botanical literature. Tables are included as a quick reference for the researcher who wishes to circumvent calculation. A Microsoft Excel 2000 spreadsheet (titled HaploPro.xls) for performing some of the more complicated calculations is offered online. Finally, the limitations of these methods and their applicability to nuclear DNA and other characters studies are discussed.
Návaznosti
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Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum biodiverzity
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Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase
VytisknoutZobrazeno: 20. 5. 2024 17:02