Characterization of Lactococcus lactis subsp. lactis isolated
from surface waters

a 2007

Characterization of Lactococcus lactis subsp. lactis isolated from surface waters

ŠVEC, Pavel a Ivo SEDLÁČEK

Základní údaje

Originální název

Characterization of Lactococcus lactis subsp. lactis isolated from surface waters

Název česky

Charakteristika Lactococcus lactis subsp. lactis izolovaných z povrchových vod

Autoři

ŠVEC, Pavel (203 Česká republika, garant) a Ivo SEDLÁČEK (203 Česká republika)

Vydání

Proceedins Connections between Collections, The 11th International Conference on Culture Collections (ICCC11), 2007

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/07:00022761

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-3-00-022417-1

Klíčová slova anglicky

Lactococcus lactis subsp. lactis; udentification; water

Štítky

Lactococcus lactis subsp. lactis, udentification, water

Změněno: 24. 3. 2010 13:11, doc. RNDr. Pavel Švec, Ph.D.

Anotace

ORIG CZ

V originále

A group of nine strains isolated on Slanetz-Bartley agar and/or Kanamycin esculin azide agar were isolated as presumptive enterococci during a screening of Enterococcus spp. in surface waters. All analysed strains were clearly separated using rep-PCR with the (GTG)5 primer from all enterococci included in the in-house CCM database but they formed a homogeneous cluster matching Lactococcus lactis subsp. lactis representatives. Further identification using extensive biotyping as well as automated ribotyping with EcoRI (RiboPrinter microbial characterization system) confirmed all strains as L. lactis subsp. lactis. Obtained results showed that L. lactis subsp. lactis can be commonly isolated from different surface waters. These strains can be confused with enterococci due to their good growth on enterococcal selective media and their similarity in key phenotypical tests. (GTG)5-PCR revealed as a reliable and fast method for identification of L. lactis subsp. lactis strains while automated ribotyping with EcoRI proved to be a good tool for intraspecies typing purposes. Financial support from project MSM0021622416 is gratefully acknowledged.

Česky

Charakteristika Lactococcus lactis subsp. lactis izolovaných z povrchových vod

Návaznosti

MSM0021622416, záměr

Název: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Diverzita biotických společenstev: kauzální analýza variability v prostoru a čase

Citovat

ŠVEC, Pavel a Ivo SEDLÁČEK. Characterization of Lactococcus lactis subsp. lactis isolated from surface waters. In Proceedins Connections between Collections, The 11th International Conference on Culture Collections (ICCC11). 2007. ISBN 978-3-00-022417-1.

@proceedings{726577,
   author = {Švec, Pavel and Sedláček, Ivo},
   booktitle = {Proceedins Connections between Collections, The 11th International Conference on Culture Collections (ICCC11)},
   keywords = {Lactococcus lactis subsp. lactis; udentification; water},
   language = {eng},
   isbn = {978-3-00-022417-1},
   title = {Characterization of Lactococcus lactis subsp. lactis isolated from surface waters},
   year = {2007}
}

TY  - CONF
ID  - 726577
AU  - Švec, Pavel - Sedláček, Ivo
PY  - 2007
TI  - Characterization of Lactococcus lactis subsp. lactis isolated from surface waters
SN  - 9783000224171
KW  - Lactococcus lactis subsp. lactis
KW  - udentification
KW  - water
N2  - A group of nine strains isolated on Slanetz-Bartley agar and/or Kanamycin esculin azide agar were isolated as presumptive enterococci during a screening of Enterococcus spp. in surface waters. All analysed strains were clearly separated using rep-PCR with the (GTG)5 primer from all enterococci included in the in-house CCM database but they formed a homogeneous cluster matching Lactococcus lactis subsp. lactis representatives. Further identification using extensive biotyping as well as automated ribotyping with EcoRI (RiboPrinter microbial characterization system) confirmed all strains as L. lactis subsp. lactis. Obtained results showed that L. lactis subsp. lactis can be commonly isolated from different surface waters. These strains can be confused with enterococci due to their good growth on enterococcal selective media and their similarity in key phenotypical tests. (GTG)5-PCR revealed as a reliable and fast method for identification of L. lactis subsp. lactis strains while automated ribotyping with EcoRI proved to be a good tool for intraspecies typing purposes. Financial support from project MSM0021622416 is gratefully acknowledged.
ER  -

ŠVEC, Pavel a Ivo SEDLÁČEK. Characterization of Lactococcus lactis subsp. lactis isolated from surface waters. In \textit{Proceedins Connections between Collections, The 11th International Conference on Culture Collections (ICCC11)}. 2007. ISBN~978-3-00-022417-1.

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