Association of miR-21, miR-31, miR-143, miR-145 and let-7a-1
levels with histopathologic features of colorectal cancer

J 2007

Association of miR-21, miR-31, miR-143, miR-145 and let-7a-1 levels with histopathologic features of colorectal cancer

SLABÝ, Ondřej, Marek SVOBODA, Pavel FABIAN, Miroslav SVOBODA, Ingrid GARAJOVÁ et. al.

Základní údaje

Originální název

Association of miR-21, miR-31, miR-143, miR-145 and let-7a-1 levels with histopathologic features of colorectal cancer

Název česky

Association of miR-21, miR-31, miR-143, miR-145 and let-7a-1 levels with histopathologic features of colorectal cancer

Autoři

SLABÝ, Ondřej (203 Česká republika), Marek SVOBODA (203 Česká republika), Pavel FABIAN (203 Česká republika), Miroslav SVOBODA (203 Česká republika), Ingrid GARAJOVÁ (703 Slovensko), Milana ŠACHLOVÁ (203 Česká republika), Tamara ŠMERDOVÁ (203 Česká republika), Dana KNOFLÍČKOVÁ (203 Česká republika) a Rostislav VYZULA (203 Česká republika, garant)

Vydání

European Journal of Cancer, 2007, 0959-8049

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.454

Kód RIV

RIV/00216224:14110/07:00019108

Organizační jednotka

Lékařská fakulta

UT WoS

000250204000249

Klíčová slova anglicky

colorectal cancer; microRNA; pathogenesis; metastasis

Štítky

Colorectal cancer, metastasis, microRNA, pathogenesis

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 1. 2008 15:34, prof. RNDr. Ondřej Slabý, Ph.D.

Anotace

ORIG CZ

V originále

RNAs, that repress protein translation through binding to target mRNAs. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). MATERIALS AND METHODS: In our study, we examined by Real-Time PCR expression levels of miR-21, miR-31, miR-143, miR-145 and let-7a-1 in bioptic samples of 29 colorectal cancer patients including 3 cases of IUCC Stage I, 11 of Stage II, 6 of Stage III, 9 of Stage IV. For 6 cases of CRC samples also adjacent non-tumor tissues were analyzed. MiRNAs expression levels were correlated with tumor stage, grade, size, anatomical localization, serum CEA levels and p53 protein expression in tumors. For data normalization we tried different approaches (18S rRNA, GAPDH, let-7a-1). Finally, variability of let-7a-1 expression was shown to be the lowest. P values were calculated using Mann-Whitney U test. RESULTS: Expression levels of all analyzed miRNAs significantly differ in tumor and normal mucosa, miR-21 (p=0,0001) and miR-31 (p=0,0006) were up-regulated and miR-143 (p=0,013) and miR-145 (p=0,018) were down-regulated in tumors. MiR-21 was also correlated with CRC stage. Although the highest levels of miR-143 and miR-145 were in normal mucosa, we identified positive correlation of tumor stage and their expression suggesting altered tumor suppressor function of these miRNAs in early events of colorectal carcinogenesis. Distal CRC showed significant up-regulation of miR-31 (p=0,024) expression. Higher serum levels of CEA were associated with down-regulation of miR-145 (p=0,05). Tumors with high expression of p53 protein had significantly lower expression of miR-143 (p=0,02). We have not associated any of studied miRNAs to tumor grade and tumor size. Tumors with down-regulated miR-143 and miR-145 were bigger and more frequent (not significantly) in proximal colon. CONCLUSIONS: Our results suggest possible roles of miR-21, miR-31, miR-143 and miR-145 in colorectal cancer pathogenesis and different histopathologic phenotypes. Supported by IGA MZ CR NR/9076 - 4

Česky

Association of miR-21, miR-31, miR-143, miR-145 and let-7a-1 levels with histopathologic features of colorectal cancer

Návaznosti

NR9076, projekt VaV

Název: Genomické profilování v predikci odpovědi na chemoradioterapii u pacientů s lokálně pokročilým karcinomem konečníku

Citovat

SLABÝ, Ondřej, Marek SVOBODA, Pavel FABIAN, Miroslav SVOBODA, Ingrid GARAJOVÁ, Milana ŠACHLOVÁ, Tamara ŠMERDOVÁ, Dana KNOFLÍČKOVÁ a Rostislav VYZULA. Association of miR-21, miR-31, miR-143, miR-145 and let-7a-1 levels with histopathologic features of colorectal cancer. European Journal of Cancer. 2007, roč. 5, 4 (suppl.), s. 78-79. ISSN 0959-8049.

@article{726890,
   author = {Slabý, Ondřej and Svoboda, Marek and Fabian, Pavel and Svoboda, Miroslav and Garajová, Ingrid and Šachlová, Milana and Šmerdová, Tamara and Knoflíčková, Dana and Vyzula, Rostislav},
   article_number = {4 (suppl.)},
   keywords = {colorectal cancer; microRNA; pathogenesis; metastasis},
   language = {eng},
   issn = {0959-8049},
   journal = {European Journal of Cancer},
   title = {Association of miR-21, miR-31, miR-143, miR-145 and let-7a-1 levels with histopathologic features of colorectal cancer},
   volume = {5},
   year = {2007}
}

TY  - JOUR
ID  - 726890
AU  - Slabý, Ondřej - Svoboda, Marek - Fabian, Pavel - Svoboda, Miroslav - Garajová, Ingrid - Šachlová, Milana - Šmerdová, Tamara - Knoflíčková, Dana - Vyzula, Rostislav
PY  - 2007
TI  - Association of miR-21, miR-31, miR-143, miR-145 and let-7a-1 levels with histopathologic features of colorectal cancer
JF  - European Journal of Cancer
VL  - 5
IS  - 4 (suppl.)
SP  - 78-79
EP  - 78-79
SN  - 09598049
KW  - colorectal cancer
KW  - microRNA
KW  - pathogenesis
KW  - metastasis
N2  - RNAs, that repress protein translation through binding to target mRNAs. Although the number of verified human miRNA is still expanding, only few have been functionally described. However, emerging evidences suggest the involvement of altered regulation of miRNA in pathogenesis of cancers and these genes are thought to function as both tumours suppressor and oncogenes. Previous studies, mainly based on microarrays technology applied on colorectal cancer cell lines, showed altered expression levels of several miRNAs in colorectal cancer (CRC). MATERIALS AND METHODS: In our study, we examined by Real-Time PCR expression levels of miR-21, miR-31, miR-143, miR-145 and let-7a-1 in bioptic samples of 29 colorectal cancer patients including 3 cases of IUCC Stage I, 11 of Stage II, 6 of Stage III, 9 of Stage IV. For 6 cases of CRC samples also adjacent non-tumor tissues were analyzed. MiRNAs expression levels were correlated with tumor stage, grade, size, anatomical localization, serum CEA levels and p53 protein expression in tumors. For data normalization we tried different approaches (18S rRNA, GAPDH, let-7a-1). Finally, variability of let-7a-1 expression was shown to be the lowest. P values were calculated using Mann-Whitney U test. RESULTS: Expression levels of all analyzed miRNAs significantly differ in tumor and normal mucosa, miR-21 (p=0,0001) and miR-31 (p=0,0006) were up-regulated and miR-143 (p=0,013) and miR-145 (p=0,018) were down-regulated in tumors. MiR-21 was also correlated with CRC stage. Although the highest levels of miR-143 and miR-145 were in normal mucosa, we identified positive correlation of tumor stage and their expression suggesting altered tumor suppressor function of these miRNAs in early events of colorectal carcinogenesis. Distal CRC showed significant up-regulation of miR-31 (p=0,024) expression. Higher serum levels of CEA were associated with down-regulation of miR-145 (p=0,05). Tumors with high expression of p53 protein had significantly lower expression of miR-143 (p=0,02). We have not associated any of studied miRNAs to tumor grade and tumor size. Tumors with down-regulated miR-143 and miR-145 were bigger and more frequent (not significantly) in proximal colon. CONCLUSIONS: Our results suggest possible roles of miR-21, miR-31, miR-143 and miR-145 in colorectal cancer pathogenesis and different histopathologic phenotypes. Supported by IGA MZ CR NR/9076 - 4
ER  -

SLABÝ, Ondřej, Marek SVOBODA, Pavel FABIAN, Miroslav SVOBODA, Ingrid GARAJOVÁ, Milana ŠACHLOVÁ, Tamara ŠMERDOVÁ, Dana KNOFLÍČKOVÁ a Rostislav VYZULA. Association of miR-21, miR-31, miR-143, miR-145 and let-7a-1 levels with histopathologic features of colorectal cancer. \textit{European Journal of Cancer}. 2007, roč.~5, 4 (suppl.), s.~78-79. ISSN~0959-8049.

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