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@article{727102,
author = {Ito, M. and Prokop, Zbyněk and Klvaňa, Martin and Otsubo, Y. and Tsuda, M. and Damborský, Jiří and Nagata, Yuji},
article_number = {4},
keywords = {hexachlorocyclohexane (HCH); Sphingobium japonicum UT26; Sphingobium sp. MI1205; haloalkane dehalogenase LinB},
language = {eng},
issn = {0302-8933},
journal = {ARCHIVES IN MICROBIOLOGY},
title = {Degradation of Beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Hexachlorocyclohexane-Utilizing Bacterium Sphingobium sp. MI1205},
url = {http://loschmidt.chemi.muni.cz/peg/abstracts/archmicro07.html},
volume = {188},
year = {2007}
}
TY - JOUR
ID - 727102
AU - Ito, M. - Prokop, Zbyněk - Klvaňa, Martin - Otsubo, Y. - Tsuda, M. - Damborský, Jiří - Nagata, Yuji
PY - 2007
TI - Degradation of Beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Hexachlorocyclohexane-Utilizing Bacterium Sphingobium sp. MI1205
JF - ARCHIVES IN MICROBIOLOGY
VL - 188
IS - 4
SP - 313-325
EP - 313-325
SN - 03028933
KW - hexachlorocyclohexane (HCH)
KW - Sphingobium japonicum UT26
KW - Sphingobium sp. MI1205
KW - haloalkane dehalogenase LinB
UR - http://loschmidt.chemi.muni.cz/peg/abstracts/archmicro07.html
N2 - The technical formulation of hexachlorocyclohexane (HCH) mainly consists of the insecticidal gamma-isomer and noninsecticidal alpha, beta, and delta-isomers, among which beta-HCH is the most recalcitrant and has caused serious environmental problems. A gamma-HCH-utilizing bacterial strain, Sphingobium sp. MI1205, was isolated from soil which had been contaminated with HCH isomers. This strain degraded beta-HCH more rapidly than the well-characterized gamma-HCH-utilizing strain Sphingobium japonicum UT26. In MI1205, beta-HCH was converted to 2,3,5,6-tetrachlorocyclohexane-1,4-diol (TCDL) via 2,3,4,5,6-pentachlorocyclohexanol (PCHL). A haloalkane dehalogenase LinB (LinB-MI) that is 98 % identical (seven amino-acid differences among 296 amino acids) to LinB from UT26 (LinB-UT) was identified as an enzyme responsible for the two-step conversion of beta-HCH to TCDL. This property of LinB-MI contrasted with that of LinB-UT, which catalyzed only the first step conversion of beta-HCH to PCHL. Site-directed mutagenesis and computer modeling suggested that two of the seven different amino acid residues (V134 and H247) forming a catalytic pocket of LinB are important for the binding of PCHL in an orientation suitable for the reaction in LinB-MI. However, mutagenesis also indicated the involvement of other residues for the activity unique to LinB-MI. Sequence analysis revealed that MI1205 possesses the IS6100-flanked cluster that contains two copies of the linB-MI gene. This cluster is identical to the one located on the exogenously isolated plasmid pLB1, suggesting that MI1205 had recruited the linB genes by a horizontal transfer event.
ER -
ITO, M., Zbyněk PROKOP, Martin KLVAŇA, Y. OTSUBO, M. TSUDA, Jiří DAMBORSKÝ a Yuji NAGATA. Degradation of Beta-Hexachlorocyclohexane by Haloalkane Dehalogenase LinB from Hexachlorocyclohexane-Utilizing Bacterium Sphingobium sp. MI1205. \textit{ARCHIVES IN MICROBIOLOGY}. 2007, roč.~188, č.~4, s.~313-325. ISSN~0302-8933.
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