Weak Activity of Haloalkane Dehalogenase LinB with
1,2,3-Trichloropropane Revealed by X-ray Crystallography and
Microcalorimetry

J 2007

Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry

MONINCOVÁ, Marta, Zbyněk PROKOP, Jitka VEVODOVÁ, Yuji NAGATA, Jiří DAMBORSKÝ et. al.

Základní údaje

Originální název

Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry

Název česky

Slabá aktivita haloalkan dehalogenázy LinB s 1,2,3-Trichloropropanem srovnávání pomocí X-ray Krystalografie a Microkalorimetrie.

Autoři

MONINCOVÁ, Marta (203 Česká republika), Zbyněk PROKOP (203 Česká republika), Jitka VEVODOVÁ (203 Česká republika), Yuji NAGATA (392 Japonsko) a Jiří DAMBORSKÝ (203 Česká republika, garant)

Vydání

Applied and Environmental Microbiology, 2007, 0099-2240

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.004

Kód RIV

RIV/00216224:14310/07:00022815

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000245156800039

Klíčová slova anglicky

1;2;3-trichloropropane (TCP); Haloalkane dehalogenases; LinB from Sphingobium japonicum UT26; X-ray crystallography; microcalorimetry

Štítky

1, 2, 3-trichloropropane (TCP), Haloalkane dehalogenases, microcalorimetry, X-ray crystallography

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 3. 2010 12:25, prof. Mgr. Jiří Damborský, Dr.

Anotace

ORIG CZ

V originále

,2,3-trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes catalysing the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP since the reaction can not be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (kcat = 0.005 s-1) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for development of new biocatalyst towards TCP by protein engineering. Microcalorimetry is proposed to be a universal method for detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering of novel biocatalysts using the scaffolds of proteins with promiscuous activities.

Česky

xxx

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

MONINCOVÁ, Marta, Zbyněk PROKOP, Jitka VEVODOVÁ, Yuji NAGATA a Jiří DAMBORSKÝ. Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry. Applied and Environmental Microbiology. 2007, roč. 73, č. 6, s. 2005-2008. ISSN 0099-2240.

@article{727112,
   author = {Monincová, Marta and Prokop, Zbyněk and Vevodová, Jitka and Nagata, Yuji and Damborský, Jiří},
   article_number = {6},
   keywords = {1;2;3-trichloropropane (TCP); Haloalkane dehalogenases; LinB from Sphingobium japonicum UT26; X-ray crystallography; microcalorimetry},
   language = {eng},
   issn = {0099-2240},
   journal = {Applied and Environmental Microbiology},
   title = {Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry},
   url = {http://loschmidt.chemi.muni.cz/peg/abstracts/aem07a.html},
   volume = {73},
   year = {2007}
}

TY  - JOUR
ID  - 727112
AU  - Monincová, Marta - Prokop, Zbyněk - Vevodová, Jitka - Nagata, Yuji - Damborský, Jiří
PY  - 2007
TI  - Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry
JF  - Applied and Environmental Microbiology
VL  - 73
IS  - 6
SP  - 2005-2008
EP  - 2005-2008
SN  - 00992240
KW  - 1;2;3-trichloropropane (TCP)
KW  - Haloalkane dehalogenases
KW  - LinB from Sphingobium japonicum UT26
KW  - X-ray crystallography
KW  - microcalorimetry
UR  - http://loschmidt.chemi.muni.cz/peg/abstracts/aem07a.html
N2  - ,2,3-trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes catalysing the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP since the reaction can not be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (kcat = 0.005 s-1) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for development of new biocatalyst towards TCP by protein engineering. Microcalorimetry is proposed to be a universal method for detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering of novel biocatalysts using the scaffolds of proteins with promiscuous activities.
ER  -

MONINCOVÁ, Marta, Zbyněk PROKOP, Jitka VEVODOVÁ, Yuji NAGATA a Jiří DAMBORSKÝ. Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry. \textit{Applied and Environmental Microbiology}. 2007, roč.~73, č.~6, s.~2005-2008. ISSN~0099-2240.

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