| MONINCOVÁ, Marta, Zbyněk PROKOP, Jitka VEVODOVÁ, Yuji NAGATA a Jiří DAMBORSKÝ. Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry. Applied and Environmental Microbiology. 2007, roč. 73, č. 6, s. 2005-2008. ISSN 0099-2240. |
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@article{727112,
author = {Monincová, Marta and Prokop, Zbyněk and Vevodová, Jitka and Nagata, Yuji and Damborský, Jiří},
article_number = {6},
keywords = {1;2;3-trichloropropane (TCP); Haloalkane dehalogenases; LinB from Sphingobium japonicum UT26; X-ray crystallography; microcalorimetry},
language = {eng},
issn = {0099-2240},
journal = {Applied and Environmental Microbiology},
title = {Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry},
url = {http://loschmidt.chemi.muni.cz/peg/abstracts/aem07a.html},
volume = {73},
year = {2007}
}
TY - JOUR
ID - 727112
AU - Monincová, Marta - Prokop, Zbyněk - Vevodová, Jitka - Nagata, Yuji - Damborský, Jiří
PY - 2007
TI - Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry
JF - Applied and Environmental Microbiology
VL - 73
IS - 6
SP - 2005-2008
EP - 2005-2008
SN - 00992240
KW - 1;2;3-trichloropropane (TCP)
KW - Haloalkane dehalogenases
KW - LinB from Sphingobium japonicum UT26
KW - X-ray crystallography
KW - microcalorimetry
UR - http://loschmidt.chemi.muni.cz/peg/abstracts/aem07a.html
N2 - ,2,3-trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes catalysing the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP since the reaction can not be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (kcat = 0.005 s-1) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for development of new biocatalyst towards TCP by protein engineering. Microcalorimetry is proposed to be a universal method for detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering of novel biocatalysts using the scaffolds of proteins with promiscuous activities.
ER -
MONINCOVÁ, Marta, Zbyněk PROKOP, Jitka VEVODOVÁ, Yuji NAGATA a Jiří DAMBORSKÝ. Weak Activity of Haloalkane Dehalogenase LinB with 1,2,3-Trichloropropane Revealed by X-ray Crystallography and Microcalorimetry. \textit{Applied and Environmental Microbiology}. 2007, roč.~73, č.~6, s.~2005-2008. ISSN~0099-2240.
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