Enzymatic reaction coupled with flow-injection analysis with
charged aerosol, coulometric, or amperometric detection for
estimation of contamination of the environment by pesticides

J 2008

Enzymatic reaction coupled with flow-injection analysis with charged aerosol, coulometric, or amperometric detection for estimation of contamination of the environment by pesticides

MIKELOVÁ, Radka, Zbyněk PROKOP, Karel STEJSKAL, Vojtěch ADAM, Miroslava BEKLOVÁ et. al.

Základní údaje

Originální název

Enzymatic reaction coupled with flow-injection analysis with charged aerosol, coulometric, or amperometric detection for estimation of contamination of the environment by pesticides

Název česky

Enzymtaická reakce ve spojení s průtokovou injekční analýzou charged aerosol, coulometrickým a amperometrickým detektorem pro stanovení kontaminace životního prostředí pesticidy

Autoři

MIKELOVÁ, Radka (203 Česká republika), Zbyněk PROKOP (203 Česká republika), Karel STEJSKAL (203 Česká republika), Vojtěch ADAM (203 Česká republika), Miroslava BEKLOVÁ (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika), Bohumila KULICHOVÁ (203 Česká republika), A. HORNA (203 Česká republika), Radka CHALOUPKOVÁ (203 Česká republika), Jiří DAMBORSKÝ (203 Česká republika, garant) a René KIZEK (203 Česká republika)

Vydání

Chromatographia, 2008, 0009-5893

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.312

Kód RIV

RIV/00216224:14310/08:00025647

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000256533700007

Klíčová slova anglicky

Halides; Coulometric; Amperometric; Pesticides

Štítky

Amperometric, Coulometric, Halides, pesticides

Změněno: 8. 7. 2009 17:53, prof. RNDr. Vojtěch Adam, Ph.D.

Anotace

V originále

Because excessive using of pesticides poses a threat to the environment and to human health, development of low-cost and sensitive methods for analysis of pesticides in the environment is needed. Several bacteria can release halide ions from the molecules of halogenated hydrocarbons. This can be used in a device for analysis of halogenated hydrocarbons in the environment by quantification of the halide anions. Here we directed our attention to selecting an instrument for detection of chloride anions. We tested three different detectors, amperometric, and coulometric, both coupled with flow-injection analysis and charged aerosol, coupled with high performance liquid chromatography. Detection limits (3 S/N) for measurement of chloride anions by use of these detectors was 30 uM (charged aerosol), 100 nM (coulometric), and 1 nM (amperometric)u. Because of its lowest detection limit for chloride anions and the many technical possibilities of miniaturization, the amperometric detector was used to test of effect of different cations on the chloride signal under the optimized experimental conditions (working electrode potential -365 mV; "Current R" 5 uA; mobile phase 0.2 M phosphate buffer, pH 6; flow rate 0.5 mL min-1). NaCl, SrCl2, NH4Cl, and CsCl were tested as sources of chloride anions. We then used the detector to detect chloride anions catalytically cleaved from 1-chlorohexane by the enzyme haloalkane dehalogenase LinB from the bacterium Sphingobium japonicum UT26. The activity of the enzyme increased with increasing reaction temperature until the maximum was observed at 39C. The results obtained were in good agreement with data obtained by colorimetric detection.

Návaznosti

MSM0021622412, záměr

Název: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Akronym: INCHEMBIOL)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni

Citovat

MIKELOVÁ, Radka, Zbyněk PROKOP, Karel STEJSKAL, Vojtěch ADAM, Miroslava BEKLOVÁ, Libuše TRNKOVÁ, Bohumila KULICHOVÁ, A. HORNA, Radka CHALOUPKOVÁ, Jiří DAMBORSKÝ a René KIZEK. Enzymatic reaction coupled with flow-injection analysis with charged aerosol, coulometric, or amperometric detection for estimation of contamination of the environment by pesticides. Chromatographia. 2008, roč. 67, č. 01, s. 47-53. ISSN 0009-5893.

@article{727128,
   author = {Mikelová, Radka and Prokop, Zbyněk and Stejskal, Karel and Adam, Vojtěch and Beklová, Miroslava and Trnková, Libuše and Kulichová, Bohumila and Horna, A. and Chaloupková, Radka and Damborský, Jiří and Kizek, René},
   article_number = {01},
   keywords = {Halides; Coulometric; Amperometric; Pesticides},
   language = {eng},
   issn = {0009-5893},
   journal = {Chromatographia},
   title = {Enzymatic reaction coupled with flow-injection analysis with charged aerosol, coulometric, or amperometric detection for estimation of contamination of the environment by pesticides},
   url = {http://loschmidt.chemi.muni.cz/peg/},
   volume = {67},
   year = {2008}
}

TY  - JOUR
ID  - 727128
AU  - Mikelová, Radka - Prokop, Zbyněk - Stejskal, Karel - Adam, Vojtěch - Beklová, Miroslava - Trnková, Libuše - Kulichová, Bohumila - Horna, A. - Chaloupková, Radka - Damborský, Jiří - Kizek, René
PY  - 2008
TI  - Enzymatic reaction coupled with flow-injection analysis with charged aerosol, coulometric, or amperometric detection for estimation of contamination of the environment by pesticides
JF  - Chromatographia
VL  - 67
IS  - 01
SP  - 47-53
EP  - 47-53
SN  - 00095893
KW  - Halides
KW  - Coulometric
KW  - Amperometric
KW  - Pesticides
UR  - http://loschmidt.chemi.muni.cz/peg/
N2  - Because excessive using of pesticides poses a threat to the environment and to human health, development of low-cost and sensitive methods for analysis of pesticides in the environment is needed. Several bacteria can release halide ions from the molecules of halogenated hydrocarbons. This can be used in a device for analysis of halogenated hydrocarbons in the environment by quantification of the halide anions. Here we directed our attention to selecting an instrument for detection of chloride anions. We tested three different detectors, amperometric, and coulometric, both coupled with flow-injection analysis and charged aerosol, coupled with high performance liquid chromatography. Detection limits (3 S/N) for measurement of chloride anions by use of these detectors was 30 uM (charged aerosol), 100 nM (coulometric), and 1 nM (amperometric)u. Because of its lowest detection limit for chloride anions and the many technical possibilities of miniaturization, the amperometric detector was used to test of effect of different cations on the chloride signal under the optimized experimental conditions (working electrode potential -365 mV; "Current R" 5 uA; mobile phase 0.2 M phosphate buffer, pH 6; flow rate 0.5 mL min-1). NaCl, SrCl2, NH4Cl, and CsCl were tested as sources of chloride anions. We then used the detector to detect chloride anions catalytically cleaved from 1-chlorohexane by the enzyme haloalkane dehalogenase LinB from the bacterium Sphingobium japonicum UT26. The activity of the enzyme increased with increasing reaction temperature until the maximum was observed at 39C. The results obtained were in good agreement with data obtained by colorimetric detection.
ER  -

MIKELOVÁ, Radka, Zbyněk PROKOP, Karel STEJSKAL, Vojtěch ADAM, Miroslava BEKLOVÁ, Libuše TRNKOVÁ, Bohumila KULICHOVÁ, A. HORNA, Radka CHALOUPKOVÁ, Jiří DAMBORSKÝ a René KIZEK. Enzymatic reaction coupled with flow-injection analysis with charged aerosol, coulometric, or amperometric detection for estimation of contamination of the environment by pesticides. \textit{Chromatographia}. 2008, roč.~67, č.~01, s.~47-53. ISSN~0009-5893.

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