| KONEČNÝ, Jiří, Ivana MIČÍKOVÁ, Roman ŘEMÍNEK a Zdeněk GLATZ. Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction. Journal of Chromatography A. Amsterdam: Elsevier, 2008, roč. 1189, 1-2, s. 244-247. ISSN 0021-9673. |
Další formáty:
BibTeX
LaTeX
RIS
@article{727227,
author = {Konečný, Jiří and Mičíková, Ivana and Řemínek, Roman and Glatz, Zdeněk},
article_location = {Amsterdam},
article_number = {1-2},
keywords = {Drug metabolism; cytochrome P450; CYP2C9; diclofenac; sulfaphenazole; ketoconazole; MEKC},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction},
volume = {1189},
year = {2008}
}
TY - JOUR
ID - 727227
AU - Konečný, Jiří - Mičíková, Ivana - Řemínek, Roman - Glatz, Zdeněk
PY - 2008
TI - Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction
JF - Journal of Chromatography A
VL - 1189
IS - 1-2
SP - 244-247
EP - 244-247
PB - Elsevier
SN - 00219673
KW - Drug metabolism
KW - cytochrome P450
KW - CYP2C9
KW - diclofenac
KW - sulfaphenazole
KW - ketoconazole
KW - MEKC
N2 - The major aim of this work is to demonstrate the applicability of micellar electrokinetic capillary chromatography with SDS based pseudostationary phase for the screening of cytochrome P450 inhibitors. In contrast with the other capillary electrophoresis modes the cytochrome P450 reaction mixture thus could be used for the analysis without any pre-treatment. Cytochrome P450 2C9, one of the most important isoforms in human liver, was chosen as a model example for this study in combination with diclofenac as a probe substrate. The inhibitory effect on the given cytochrome P450 reaction was evaluated for two inhibitors with different inhibition potential strong inhibitor sulfaphenazole and moderate inhibitor ketoconazole. As a result 50 % inhibitory concentrations IC50 and inhibition constants Ki were evaluated; their values for both inhibitors were in a good agreement with the literature data determined by different methods.
ER -
KONEČNÝ, Jiří, Ivana MIČÍKOVÁ, Roman ŘEMÍNEK a Zdeněk GLATZ. Application of Micellar Electrokinetic Capillary Chromatography for Evaluation of Inhibitory Effects on Cytochrome P450 Reaction. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2008, roč.~1189, 1-2, s.~244-247. ISSN~0021-9673.
|
