Serological Diagnosis of Tularemia in Mice Using the
Amperometric Immunosensor

J 2007

Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor

POHANKA, Miroslav a Petr SKLÁDAL

Základní údaje

Originální název

Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor

Název česky

Serologické stanovení tularemie u myší použitím amperometrického imunosensoru

Autoři

POHANKA, Miroslav (203 Česká republika) a Petr SKLÁDAL (203 Česká republika, garant)

Vydání

Electroanalysis, Weinheim, Wiley-VCH Verlag GmbH, 2007, 1040-0397

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.949

Kód RIV

RIV/00216224:14310/07:00022879

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000251891400003

Klíčová slova anglicky

Amperometric biosensor; Screen-printed sensor; Immunoassay; Francisella tularensis

Štítky

amperometric biosensor, Francisella tularensis, Immunoassay, Screen-printed sensor

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 31. 1. 2008 23:10, prof. RNDr. Petr Skládal, CSc.

Anotace

ORIG CZ

V originále

Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day, it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections.

Česky

Bylo detekováno množství anti tularemických protilátek v séru myší infikovaných bakterií Francisella tularensis, pro stanovení byl využit amperometrický imunosensor na bázi sítotiskové elektrody.

Návaznosti

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

Citovat

POHANKA, Miroslav a Petr SKLÁDAL. Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor. Electroanalysis. Weinheim: Wiley-VCH Verlag GmbH, 2007, roč. 19, č. 23, s. 2507-2512. ISSN 1040-0397.

@article{727506,
   author = {Pohanka, Miroslav and Skládal, Petr},
   article_location = {Weinheim},
   article_number = {23},
   keywords = {Amperometric biosensor; Screen-printed sensor; Immunoassay; Francisella tularensis},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   note = {DOI 10.1002/elan.200704003},
   title = {Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor},
   url = {http://www3.interscience.wiley.com/cgi-bin/abstract/116320027/ABSTRACT?CRETRY=1&SRETRY=0},
   volume = {19},
   year = {2007}
}

TY  - JOUR
ID  - 727506
AU  - Pohanka, Miroslav - Skládal, Petr
PY  - 2007
TI  - Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor
JF  - Electroanalysis
VL  - 19
IS  - 23
SP  - 2507-2512
EP  - 2507-2512
PB  - Wiley-VCH Verlag GmbH
SN  - 10400397
N1  - DOI 10.1002/elan.200704003
KW  - Amperometric biosensor
KW  - Screen-printed sensor
KW  - Immunoassay
KW  - Francisella tularensis
UR  - http://www3.interscience.wiley.com/cgi-bin/abstract/116320027/ABSTRACT?CRETRY=1&SRETRY=0
N2  - Tularemia caused by the bacterium Francisella tularensis belongs to danger infective diseases, therefore timely diagnosis is an important part of protective activities. A novel approach for serological diagnostics of tularemia using a four-channel amperometric immunosensor was proposed and evaluated on BALB/c mice as a model organism. The immunosensor was able to work with small volumes of serum samples (0.05 L per assay) and diagnosed tularemic infection very early after immunization. From the 5th day, it was possible to distinguish tularemic sera from control sera from mice immunized with Escherichia coli on the probability level of 0.99 (t-test). Some 40 measurements per one hour can be realized with the developed procedure. The obtained results were confirmed by the standard indirect ELISA. The proposed immunosensor-based approach seems promising for rapid detection of microbial pathogen infections.
ER  -

POHANKA, Miroslav a Petr SKLÁDAL. Serological Diagnosis of Tularemia in Mice Using the Amperometric Immunosensor. \textit{Electroanalysis}. Weinheim: Wiley-VCH Verlag GmbH, 2007, roč.~19, č.~23, s.~2507-2512. ISSN~1040-0397.

Podrobný výpis o publikaci