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@article{745025, author = {Bártová, Eva and Pacherník, Jiří and Kozubík, Alois and Kozubek, Stanislav}, article_location = {Berlin / Heidelberg}, article_number = {4}, keywords = {HP1 proteins - TIF1beta - Chromocentres - Differentiation - Embryonal carcinoma cells}, issn = {0948-6143}, journal = {Histochem Cell Biol.}, title = {Differentiation-specific association of HP1alpha and HP1beta with chromocentres is correlated with clustering of TIF1beta at these sites.}, volume = {127}, year = {2007} }
TY - JOUR ID - 745025 AU - Bártová, Eva - Pacherník, Jiří - Kozubík, Alois - Kozubek, Stanislav PY - 2007 TI - Differentiation-specific association of HP1alpha and HP1beta with chromocentres is correlated with clustering of TIF1beta at these sites. JF - Histochem Cell Biol. VL - 127 IS - 4 SP - 375-388 EP - 375-388 PB - Springer SN - 09486143 KW - HP1 proteins - TIF1beta - Chromocentres - Differentiation - Embryonal carcinoma cells N2 - Mammalian heterochromatin protein 1 (HP1alpha, HP1beta, HP1gama subtypes) and transcriptional intermediary factor TIF1beta play an important role in the regulation of chromatin structure and function. Here, we investigated the nuclear arrangement of these proteins during differentiation of embryonal carcinoma P19 cells into primitive endoderm and into the neural pathway. Additionally, the differentiation potential of trichostatin A (TSA) and 5-deoxyazacytidine (5-dAzaC) was studied. In 70% of the cells from the neural pathway and in 20% of TSA-stimulated cells, HP1alpha and HP1beta co-localized and associated with chromocentres (clusters of centromeres), which correlated with clustering of TIF1beta at these heterochromatic regions. The cell types that we studied were also characterized by a pronounced focal distribution of HP1gama. The above-mentioned nuclear patterns of HP1 and TIF1beta proteins were completely different from the nuclear patterns observed in the remaining cell types investigated, in which HP1alpha was associated with chromocentres while HP1beta and HP1gama were largely localized in distinct nuclear regions. Moreover, a dispersed nuclear distribution of TIF1beta was observed. Our findings showed that the nuclear arrangement of HP1 subtypes and TIF1beta is differentiation specific, and seems to be more important than changes in the levels of these proteins, which were relatively stable during all the induced differentiation processes. ER -
BÁRTOVÁ, Eva. Differentiation-specific association of HP1alpha and HP1beta with chromocentres is correlated with clustering of TIF1beta at these sites. \textit{Histochem Cell Biol.}. Berlin / Heidelberg: Springer, 2007, vol.~127, No~4, p.~375-388. ISSN~0948-6143.
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