Distinct nuclear arrangement of active and inactive c-myc genes
in control and differentiated colon carcinoma cells.

J 2006

Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.

HARNIČAROVÁ, Andrea

Základní údaje

Originální název

Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.

Autoři

HARNIČAROVÁ, Andrea

Vydání

Exp Cell Res. Elsevier, 2006, 0014-4827

Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.777

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000242838100006

Klíčová slova anglicky

Chromosome structure; c-myc gene; Gene expression; RNA-FISH, Differentiation

Štítky

c-myc gene, chromosome structure, GENE EXPRESSION, RNA-FISH, Differentiation

Změněno: 12. 12. 2007 11:13, Mgr. Jiří Pacherník, Ph.D.

Anotace

V originále

Using sequential RNA-DNA fluorescence in situ hybridization, the nuclear arrangement of both the active and inactive c-myc gene as well as its transcription was investigated in colon cancer HT-29 cells induced to differentiate into enterocytes. Cytogenetic studies revealed the presence of two chromosomes 8 in HT-29 cells, of which the one containing c-myc gene amplicons was substantially larger and easily distinguished from the normal chromosome. This observation enabled detection of both activity and nuclear localization of c-myc genes in single cells and in individual chromosome territories. Similar transcriptional activity of the c-myc gene was observed in both the normal and derivative chromosome 8 territories showing no influence of the amplification on the c-myc gene expression. Our experiments demonstrate strikingly specific nuclear and territorial arrangements of active genes as compared with inactive ones: on the periphery of their territories facing to the very central region of the cell nucleus. Nuclear arrangement of c-myc genes and transcripts was conserved during cell differentiation and, therefore, independent of the level of differentiation-specific c-myc gene expression. However, after the induction of differentiation, a more internal territorial location was found for the single copy c-myc gene of normal chromosome 8, while amplicons conserved their territorial topography.

Citovat

HARNIČAROVÁ, Andrea. Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells. Exp Cell Res. Elsevier, 2006, roč. 312, č. 20, 4019-4035. ISSN 0014-4827.

@article{745052,
   author = {Harničarová, Andrea and Kozubek, Stanislav and Pacherník, Jiří and Krejčí, Jana and Bártová, Eva},
   article_number = {20},
   keywords = {Chromosome structure; c-myc gene; Gene expression; RNA-FISH, Differentiation},
   issn = {0014-4827},
   journal = {Exp Cell Res.},
   title = {Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.},
   volume = {312},
   year = {2006}
}

TY  - JOUR
ID  - 745052
AU  - Harničarová, Andrea - Kozubek, Stanislav - Pacherník, Jiří - Krejčí, Jana - Bártová, Eva
PY  - 2006
TI  - Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells.
JF  - Exp Cell Res.
VL  - 312
IS  - 20
PB  - Elsevier
SN  - 00144827
KW  - Chromosome structure
KW  - c-myc gene
KW  - Gene expression
KW  - RNA-FISH, Differentiation
N2  - Using sequential RNA-DNA fluorescence in situ hybridization, the nuclear arrangement of both the active and inactive c-myc gene as well as its transcription was investigated in colon cancer HT-29 cells induced to differentiate into enterocytes. Cytogenetic studies revealed the presence of two chromosomes 8 in HT-29 cells, of which the one containing c-myc gene amplicons was substantially larger and easily distinguished from the normal chromosome. This observation enabled detection of both activity and nuclear localization of c-myc genes in single cells and in individual chromosome territories. Similar transcriptional activity of the c-myc gene was observed in both the normal and derivative chromosome 8 territories showing no influence of the amplification on the c-myc gene expression. Our experiments demonstrate strikingly specific nuclear and territorial arrangements of active genes as compared with inactive ones: on the periphery of their territories facing to the very central region of the cell nucleus. Nuclear arrangement of c-myc genes and transcripts was conserved during cell differentiation and, therefore, independent of the level of differentiation-specific c-myc gene expression. However, after the induction of differentiation, a more internal territorial location was found for the single copy c-myc gene of normal chromosome 8, while amplicons conserved their territorial topography.
ER  -

HARNIČAROVÁ, Andrea. Distinct nuclear arrangement of active and inactive c-myc genes in control and differentiated colon carcinoma cells. \textit{Exp Cell Res.}. Elsevier, 2006, roč.~312, č.~20, 4019-4035. ISSN~0014-4827.

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