2007
Bilateral changes of SDF-1 and its receptor CXCR4 in the dorsal root ganglia of chronic constriction injury and spinal nerve ligation models of neuropathic pain
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana SVÍŽENSKÁ a Radim JANČÁLEKZákladní údaje
Originální název
Bilateral changes of SDF-1 and its receptor CXCR4 in the dorsal root ganglia of chronic constriction injury and spinal nerve ligation models of neuropathic pain
Název česky
Bilaterální změny expresi SDF1 ajeho receptoru CXCR4 ve spinálních gangliích po konstrikci sedacího nervu a ligatuře spinálního nervu
Autoři
DUBOVÝ, Petr, Ilona KLUSÁKOVÁ, Ivana SVÍŽENSKÁ a Radim JANČÁLEK
Vydání
Sixth Conference of the Czech Neuroscience Society, 2007
Další údaje
Jazyk
angličtina
Typ výsledku
Prezentace na konferencích
Obor
30000 3. Medical and Health Sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Organizační jednotka
Lékařská fakulta
Klíčová slova anglicky
neuropathic pain-experimental model-Wallerian degeneration-chemokine
Příznaky
Mezinárodní význam
Změněno: 21. 1. 2008 09:13, MUDr. Ilona Klusáková, Ph.D.
V originále
There is compelling evidence indicating that hyperalgesia, allodynia and ongoing pain associated with peripheral nerve injury are due to changes in the DRG. Chronic constriction injury (CCI) and spinal nerve ligation (SNL) are most frequently used experimental models of neuropathic pain based on nerve injury. Chemokines are small chemotactic cytokines which produce their effects by activating a family of G-protein coupled receptors. Some recent studies have shown that regulation of chemokines is one of the mechanisms underlying the development and maintenance of neuropathic pain. The biological activity of stromal cell-derived factor (SDF1) is signaled through the chemokine receptor CXCR4 which is unique among chemokine receptors, having only one known ligand. The role of SDF1/CXCR4 in the peripheral nervous system is implied by their early expression in neural crest cells/derivatives as well as in the dorsal root ganglia (DRG). The goal of our experiments was to compare an immunohistochemical staining for SDF-1 and CXCR4 proteins in the L4-L5 DRG of naïve rats and those operated for unilateral L4-L5 SNL and CCI of sciatic nerve. The naive DRG displayed a sharp immunofluorescence for SDF1 (SDF1-IF) at the surface of neuron/satellite glial cell units. A significant decrease of SDF1-IF was induced bilaterally in the L4-L5 DRG 3 days after both CCI and SNL. A diffuse immunofluorescence for SDF1 was found in SGC after both types of nerve injuries for 1 and 2 weeks with a higher intensity following CCI. CXCR4-IF was present in the small- and medium sized neurons and the satellite glial cells (SGC) enveloping the large-sized neurons of naïve DRG. CXCR4-IF was unchanged in the small- and medium sized neurons in the DRG from operated rats for all periods of survival. A significant reduced CXCR4-IF was observed in the SGC of contralateral DRG after both types of nerve injuries, but was elevated at the surface of large-sized neurons of ipsilateral DRG when CCI was applied. Latter pattern of staining was not significantly developed following SNL. Our results suggest different regulation of SDF1/CXCR4 expression in the DRG during various periods of survival and by the type of nerve injury used in neuropathic pain models.
Česky
Hyperalgesie a allodynie vznikají po poškození periferních nervů na zákaldě změn, které jsou ve spinálních gangliích.
Návaznosti
| GA309/07/0121, projekt VaV |
| ||
| MSM0021622404, záměr |
|