2007
Increased invasion of ED-1 positive macrophages in both ipsi- and contralateral dorsal root ganglia following unilateral nerve injuries
DUBOVÝ, Petr, Lucie TUČKOVÁ, Radim JANČÁLEK, Ivana SVÍŽENSKÁ, Ilona KLUSÁKOVÁ et. al.Základní údaje
Originální název
Increased invasion of ED-1 positive macrophages in both ipsi- and contralateral dorsal root ganglia following unilateral nerve injuries
Název česky
Zvýšená invaze ED1 pozitivních makrofágů v ipsi- a kontralaterálních spinálních gangliích po unilaterálním poškození nervu
Autoři
DUBOVÝ, Petr (203 Česká republika, garant, domácí), Lucie TUČKOVÁ (203 Česká republika, domácí), Radim JANČÁLEK (203 Česká republika, domácí), Ivana SVÍŽENSKÁ (203 Česká republika, domácí) a Ilona KLUSÁKOVÁ (203 Česká republika, domácí)
Vydání
Neuroscience Letters, Elsevier, 2007, 0304-3940
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
30000 3. Medical and Health Sciences
Stát vydavatele
Irsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 2.085
Kód RIV
RIV/00216224:14110/07:00020667
Organizační jednotka
Lékařská fakulta
UT WoS
000251407400004
Klíčová slova anglicky
macrophage invasion; immunohistochemistry; neuropathic pain model
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 10. 1. 2012 23:50, prof. MUDr. Radim Jančálek, Ph.D., MBA
V originále
There is increasing evidence that unilateral nerve injury induces cellular and molecular changes in the associated DRG not only on the ipsilateral but also in the contralateral side. In this investigation, ED-1+ macrophages were quantified by image analysis in the naive L5 DRG (nDRG) and compared with the ipsi- and contralateral ones 2 and 4 weeks after unilateral sciatic nerve ligature and ventral root transection (VRT). A few ED-1+ macrophages were found in nDRG but not closely associated with the neuronal bodies. In contrast, following nerve injuries ED-1+ macrophages and their processes were frequently located close neuronal bodies and became their satellite cells. Moreover, an increased number of ED-1+ cells was found in the ipsilateral DRG 2 weeks after unilateral sciatic nerve ligature or VRT, but no significant differences were measured between 2 and 4 weeks after both types of nerve lesion. Contralateral DRG displayed a significant enhanced number of ED-1+ cells no sooner than 4 weeks from sciatic nerve ligature. In contrast, VRT induced a significant increased invasion of the ED-1+ cells in the contralateral DRG as early as 2 weeks after operation. Our experiments indicate that a significantly higher number of ED-1+ macrophages remained in both ipsi- and contralateral DRG up to 4 weeks from nerve injury. Based on results from different models of nerve injury, we suggest that more than one mechanism operates to stimulate the invasion of ED-1+ macrophages into the DRG including retrograde transport of factors produced during Wallerian degeneration or their delivery by blood flow. Signaling for macrophage invasion into DRG contralateral to nerve injury may be mediated by lost motoneurons or by interneurones.
Česky
Unilaterální poškození nervu způsobuje buněčné a molekulární změny ve spinálních gangliích nejen na straně poškození, ale i na kontralaterální straně.
Návaznosti
| GA309/07/0121, projekt VaV |
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| MSM0021622404, záměr |
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