MIKELOVÁ, Radka and Vojtěch ADAM. Utilizing of electrochemical detection coupled with liquid chromatography for isoflavones detection (Utilizing of electrochemical). Acta Chimica Slovenica. Slovenská republika, 2007, vol. 54, No 1, p. 92-97. ISSN 1318-0207.
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Basic information
Original name Utilizing of electrochemical detection coupled with liquid chromatography for isoflavones detection
Name in Czech Využití elektrochemické detekce spojené s kapalinovou chromatografií pro stanovení isoflavonů
Authors MIKELOVÁ, Radka and Vojtěch ADAM.
Edition Acta Chimica Slovenica, Slovenská republika, 2007, 1318-0207.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10405 Electrochemistry
Country of publisher Slovakia
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.093
Organization unit Faculty of Science
Keywords in English isoflavones; HPLC
Tags HPLC, Isoflavones, ZR
Changed by Changed by: Ing. Zdeňka Rašková, učo 140529. Changed: 16/4/2012 16:14.
Abstract
Among the biologically important roles of isoflavones is also their effect on carcinogenesis. We used flow injection analysis and high performance liquid W with electrochemical detection to simultaneously determine certain isoflavones (biochanin A, formononetin, sissotrin, daidzin, daidzein, glycitin, glycitein and genistein). The most suitable chromatographic conditions were: mobile phase: 0.2 mol L--1 acetate buffer (pH 5.0); flow rate 2.0 mL min--1; column and detector temperature: 26 C; detection potential: 800 mV. Under the optimal conditions, the detection limits were in the range of several ng mL--1. Their simultaneous determination takes 15 min.
Abstract (in Czech)
K důležité biologické roly isoflavonů patří jejich efekt na karcerogenezi. My jsme užili průtokovou injekční analýzu a vysoce účinnou kapalinoou chromatografii s elektrochhemickou detekcí pro simultánní stanovení isoflavonů (biochaninu A, formononetinu, sissotrinu, daidzinu, daidzeinu, glycitinu, glyciteinu a genisteinu. Námi použité chromatogarfické podmínky byly: mobilní fáze : 0.2 mol/l acetátvého pufru (pH 5.0), průtok 2.0 ml/min, teplota kolonového prostoru 26 st.C, detekční potenciál 800 mV. Za těchto podmínek se detekční limity pohybovali okolo ng/ml. Simultánní stanovení trvalo 15 minut.
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