Colorectal carcinoma is infiltrated by FOXP3-positive
lymphocytes

D 2007

Colorectal carcinoma is infiltrated by FOXP3-positive lymphocytes

GARAJOVÁ, Ingrid, Pavel FABIAN, Rudolf NENUTIL, Ilona KOCÁKOVÁ, Peter GRELL et. al.

Základní údaje

Originální název

Colorectal carcinoma is infiltrated by FOXP3-positive lymphocytes

Název česky

Infiltrace kolorektálního karcinomu FOXP3- pozitivními lymfocyty

Autoři

GARAJOVÁ, Ingrid, Pavel FABIAN, Rudolf NENUTIL, Ilona KOCÁKOVÁ, Peter GRELL, Zina HANZELKOVÁ, Rostislav VYZULA a Marek SVOBODA

Vydání

Florida, USA, Molecular Targets in Cancer Therapy:Mechanism and Therapeutic Reversal of Immune Suppression in Cancer, od s. 139-139, 1 s. 2007

Nakladatel

H.Lee Moffitt Center and Research Institute and NCI Comprehensive Cancer Care

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

FOXP-3 protein;T-regulatory lymphocytes;colorectal carcinoma

Štítky

colorectal carcinoma, FOXP-3 protein, T-regulatory lymphocytes

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 19. 12. 2007 01:36, prof. MUDr. Marek Svoboda, Ph.D.

Anotace

ORIG CZ

V originále

Tumor is a complex tissue composed of cancer cells and stromal cells (e.g. endothelial cells, fibroblasts, dendritic and NK cells, macrophages and lymphocytes). Tumor-infiltrating lymphocytes (TIL) are found in a variety of solid cancers and they are a possible prognostic factor as it is thought that TILs execute a host immune response against cancer cells. In colorectal carcinoma (CRC), TILS are particularly numerous in cases associated with microsatellite instability and have more favorable clinical outcome. Generally, cytotoxic T-cells (CD8+) are prognostically favorable, whereas recent discovered subgroup of TILs, regulatory T-cells (T-reg, CD4+CD25+FOXP3+) are not. They inhibit antitumor activity of CD8+ and CD4+ T-cells. The aim of our study was to investigate if the TIL of CRC include T-reg lymphocytes, which was not proved so far. More recent studies have shown that T-reg lymphocytes are unically characterized by expression of transcription factor FOXP3. Therefore we used immunohistochemical staining to detect lymphocytes co-expressing CD4+ and FOXP3+ in 9 cases of CRC primary tumors. All cases of CRC were left-side localized, respecting a different biological behaviour of left/right-side localized CRCs. We used formalin-fixed and paraffin-embedded sections and commercially available monoclonal antibodies. Our preliminary results show that TIL in CRC include in cancer stroma a subset of CD4+ FOXP3+ lymphocytes. Now, we are interested if T-reg lymphocytes can be used as a prognostic marker for CRC and we analyze a group of 40 patients with CRC in I-IV clinical stage. We are also interested if there is a connection between occurrence of T-reg and other stromal cells, especially cytotoxic T-lymphocytes and NK cells. This project is supported by Internal Grant Agency, Ministry of Health, Czech Republic. No.:NR/9076-4.

Česky

Publikace je pouze v anglickém jazyku.

Návaznosti

NR9076, projekt VaV

Název: Genomické profilování v predikci odpovědi na chemoradioterapii u pacientů s lokálně pokročilým karcinomem konečníku

Citovat

GARAJOVÁ, Ingrid, Pavel FABIAN, Rudolf NENUTIL, Ilona KOCÁKOVÁ, Peter GRELL, Zina HANZELKOVÁ, Rostislav VYZULA a Marek SVOBODA. Colorectal carcinoma is infiltrated by FOXP3-positive lymphocytes. In Molecular Targets in Cancer Therapy:Mechanism and Therapeutic Reversal of Immune Suppression in Cancer. Florida, USA: H.Lee Moffitt Center and Research Institute and NCI Comprehensive Cancer Care, 2007, s. 139-139.

@inproceedings{746149,
   author = {Garajová, Ingrid and Fabian, Pavel and Nenutil, Rudolf and Kocáková, Ilona and Grell, Peter and Hanzelková, Zina and Vyzula, Rostislav and Svoboda, Marek},
   address = {Florida, USA},
   booktitle = {Molecular Targets in Cancer Therapy:Mechanism and Therapeutic Reversal of Immune Suppression in Cancer},
   keywords = {FOXP-3 protein;T-regulatory lymphocytes;colorectal carcinoma},
   language = {eng},
   location = {Florida, USA},
   pages = {139-139},
   publisher = {H.Lee Moffitt Center and Research Institute and NCI Comprehensive Cancer Care},
   title = {Colorectal carcinoma is infiltrated by FOXP3-positive lymphocytes},
   year = {2007}
}

TY  - JOUR
ID  - 746149
AU  - Garajová, Ingrid - Fabian, Pavel - Nenutil, Rudolf - Kocáková, Ilona - Grell, Peter - Hanzelková, Zina - Vyzula, Rostislav - Svoboda, Marek
PY  - 2007
TI  - Colorectal carcinoma is infiltrated by FOXP3-positive lymphocytes
PB  - H.Lee Moffitt Center and Research Institute and NCI Comprehensive Cancer Care
CY  - Florida, USA
KW  - FOXP-3 protein;T-regulatory lymphocytes;colorectal carcinoma
N2  - Tumor is a complex tissue composed of cancer cells and stromal cells (e.g. endothelial cells, fibroblasts, dendritic and NK cells, macrophages and lymphocytes). Tumor-infiltrating lymphocytes (TIL) are found in a variety of solid cancers and they are a possible prognostic factor as it is thought that TILs execute a host immune response against cancer cells. In colorectal carcinoma (CRC), TILS are particularly numerous in cases associated with microsatellite instability and have more favorable clinical outcome. Generally, cytotoxic T-cells (CD8+) are prognostically favorable, whereas recent discovered subgroup of TILs, regulatory T-cells (T-reg, CD4+CD25+FOXP3+) are not. They inhibit antitumor activity of CD8+ and CD4+ T-cells. The aim of our study was to investigate if the TIL of CRC include T-reg lymphocytes, which was not proved so far. More recent studies have shown that T-reg lymphocytes are unically characterized by expression of transcription factor FOXP3. Therefore we used immunohistochemical staining to detect lymphocytes co-expressing CD4+ and FOXP3+ in 9 cases of CRC primary tumors. All cases of CRC were left-side localized, respecting a different biological behaviour of left/right-side localized CRCs. We used formalin-fixed and paraffin-embedded sections and commercially available monoclonal antibodies. Our preliminary results show that TIL in CRC include in cancer stroma a subset of CD4+ FOXP3+ lymphocytes. Now, we are interested if T-reg lymphocytes can be used as a prognostic marker for CRC and we analyze a group of 40 patients with CRC in I-IV clinical stage. We are also interested if there is a connection between occurrence of T-reg and other stromal cells, especially cytotoxic T-lymphocytes and NK cells. This project is supported by Internal Grant Agency, Ministry of Health, Czech Republic. No.:NR/9076-4.
ER  -

GARAJOVÁ, Ingrid, Pavel FABIAN, Rudolf NENUTIL, Ilona KOCÁKOVÁ, Peter GRELL, Zina HANZELKOVÁ, Rostislav VYZULA a Marek SVOBODA. Colorectal carcinoma is infiltrated by FOXP3-positive lymphocytes. In \textit{Molecular Targets in Cancer Therapy:Mechanism and Therapeutic Reversal of Immune Suppression in Cancer}. Florida, USA: H.Lee Moffitt Center and Research Institute and NCI Comprehensive Cancer Care, 2007, s.~139-139.

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