Lymphocyte showing the phenotype of regulatory T-cells
infiltrate colon carcinoma

a 2007

Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma

GARAJOVÁ, Ingrid, Pavel FABIAN, Rudolf NENUTIL, Ondřej SLABÝ, Ilona KOCÁKOVÁ et. al.

Základní údaje

Originální název

Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma

Název česky

Lymfocyty vykazujíci fenotyp T-regulačných buněk infiltrují karcinom střeva

Autoři

GARAJOVÁ, Ingrid (703 Slovensko), Pavel FABIAN (203 Česká republika), Rudolf NENUTIL (203 Česká republika), Ondřej SLABÝ (203 Česká republika), Ilona KOCÁKOVÁ (203 Česká republika), Peter GRELL (703 Slovensko), Zina HANZELKOVÁ (203 Česká republika), Marek SVOBODA (203 Česká republika) a Rostislav VYZULA (203 Česká republika, garant)

Vydání

Abstract book. 2007

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Itálie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/07:00019123

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

regulatory T-cells; colon carcinoma

Štítky

colon carcinoma, regulatory T-cells

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 4. 2010 14:57, prof. RNDr. Ondřej Slabý, Ph.D.

Anotace

ORIG CZ

V originále

We have previously shown that colon carcinoma (CC) is infiltrated by FOXP3-positive lymphocytes. Although the transcription factor FOXP3 is claimed to be specific for regulatory T (TREG) cells, the canonical TREG population is known to show the CD4+ CD25+ (FOXP3+) immunophenotype. The aim of the current study was therefore to investigate whether FOXP3+ cells infiltrating CC coexpress indeed CD4 and CD25 molecules. Double-staining experiments were therefore performed on serial sections obtained from formalin-fixed, paraffin-embedded specimens of nine cases of CC. The CC infiltrate included numerous CD4+ as well as CD25+ cells; moreover CD4+ cells harboured both FOXP3+ and FOXP3- lymphocytes; similarly, CD25+ cells included both FOXP3+ and FOXP3- lymphocytes. Nevertheless, all the FOXP3+ cells showed simultaneously CD4-positivity; similarly, all the FOXP3+ cells showed simultaneously CD25-positivity. Thus, the FOXP3+ lymphocytes infiltrating CC are characterized by the coexpression of both CD4 and CD25 molecules, and show therefore an immunophenotype overlapping with that of standard CD4+CD25+FOXP3+ TREG cells. The presence of lymphocytes resembling TREG cells within the microenvironment of CC can explain why the CC tumour mass, despite being also infiltrated by cytolytic anti-tumour CD8+/CD4+ lymphocytes, shows however the well known capability to undergo fatal progression. Acknowledgment: This project is supported by Internal Grant Agency, Ministry of Health, Czech Republic. No.: NR/9076-4.

Česky

Publikace je pouze v anglickém jazyku.

Návaznosti

NR9076, projekt VaV

Název: Genomické profilování v predikci odpovědi na chemoradioterapii u pacientů s lokálně pokročilým karcinomem konečníku

Citovat

GARAJOVÁ, Ingrid, Pavel FABIAN, Rudolf NENUTIL, Ondřej SLABÝ, Ilona KOCÁKOVÁ, Peter GRELL, Zina HANZELKOVÁ, Marek SVOBODA a Rostislav VYZULA. Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma. In Abstract book. 2007.

@proceedings{746221,
   author = {Garajová, Ingrid and Fabian, Pavel and Nenutil, Rudolf and Slabý, Ondřej and Kocáková, Ilona and Grell, Peter and Hanzelková, Zina and Svoboda, Marek and Vyzula, Rostislav},
   booktitle = {Abstract book.},
   keywords = {regulatory T-cells; colon carcinoma},
   language = {eng},
   title = {Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma},
   year = {2007}
}

TY  - CONF
ID  - 746221
AU  - Garajová, Ingrid - Fabian, Pavel - Nenutil, Rudolf - Slabý, Ondřej - Kocáková, Ilona - Grell, Peter - Hanzelková, Zina - Svoboda, Marek - Vyzula, Rostislav
PY  - 2007
TI  - Lymphocyte showing the phenotype of regulatory T-cells infiltrate colon carcinoma
KW  - regulatory T-cells
KW  - colon carcinoma
N2  - We have previously shown that colon carcinoma (CC) is infiltrated by FOXP3-positive lymphocytes. Although the transcription factor FOXP3 is claimed to be specific for regulatory T (TREG) cells, the canonical TREG population is known to show the CD4+ CD25+ (FOXP3+) immunophenotype. The aim of the current study was therefore to investigate whether FOXP3+ cells infiltrating CC coexpress indeed CD4 and CD25 molecules. Double-staining experiments were therefore performed on serial sections obtained from formalin-fixed, paraffin-embedded specimens of nine cases of CC. The CC infiltrate included numerous CD4+ as well as CD25+ cells; moreover CD4+ cells harboured both FOXP3+ and FOXP3- lymphocytes; similarly, CD25+ cells included both FOXP3+ and FOXP3- lymphocytes. Nevertheless, all the FOXP3+ cells showed simultaneously CD4-positivity; similarly, all the FOXP3+ cells showed simultaneously CD25-positivity. Thus, the FOXP3+ lymphocytes infiltrating CC are characterized by the coexpression of both CD4 and CD25 molecules, and show therefore an immunophenotype overlapping with that of standard CD4+CD25+FOXP3+ TREG cells. The presence of lymphocytes resembling TREG cells within the microenvironment of CC can explain why the CC tumour mass, despite being also infiltrated by cytolytic anti-tumour CD8+/CD4+ lymphocytes, shows however the well known capability to undergo fatal progression. Acknowledgment: This project is supported by Internal Grant Agency, Ministry of Health, Czech Republic. No.: NR/9076-4.
ER  -

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