| KRYŠTOFOVÁ, Olga, Jan HRADECKÝ, Josef ZEHNÁLEK, Ladislav HAVEL, Libuše TRNKOVÁ a René KIZEK. Comparison of spectrophotometry to electrochemical methods of urease determination. In Chemické zvesti. Slovenská republika: Institute of Chemistry Slovak Acedemy of Science, Bratislava, Slovak Chemical Society, Bratislava, 2007, s. 169-169. ISSN 1336-7242. |
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@inproceedings{746855,
author = {Kryštofová, Olga and Hradecký, Jan and Zehnálek, Josef and Havel, Ladislav and Trnková, Libuše and Kizek, René},
address = {Slovenská republika},
booktitle = {Chemické zvesti},
keywords = {Electrochemistry; Spectrophotometry; Urease},
language = {eng},
location = {Slovenská republika},
pages = {169-169},
publisher = {Institute of Chemistry Slovak Acedemy of Science, Bratislava, Slovak Chemical Society, Bratislava},
title = {Comparison of spectrophotometry to electrochemical methods of urease determination},
year = {2007}
}
TY - JOUR
ID - 746855
AU - Kryštofová, Olga - Hradecký, Jan - Zehnálek, Josef - Havel, Ladislav - Trnková, Libuše - Kizek, René
PY - 2007
TI - Comparison of spectrophotometry to electrochemical methods of urease determination
PB - Institute of Chemistry Slovak Acedemy of Science, Bratislava, Slovak Chemical Society, Bratislava
CY - Slovenská republika
KW - Electrochemistry
KW - Spectrophotometry
KW - Urease
N2 - Enzyme urease is ubiquitous enzyme in plants and is also present in many eucaryote microorganisms and bacterias. It consists of 840 aminoacids residues and molecular weight is higher than 90 kDa. It playse a key role in catalysis of dissociation of urea while the formation of carbon dioxide and ammonia. For enzyme activity analysis was developed a series of direct and indirect analytical methods. The most used are the detection of ammonia by reaction with indophenol, determination 14CO2 and series of enzymatic reactions coupled with spectrophotometric measurement of NADPH oxidation. The aim of this study was to compare spectrophotometric detection of urease activity completed by Berthelot reaction and electrochemical detection of reduction signals of urease and Brdička catalytic signals.
ER -
KRYŠTOFOVÁ, Olga, Jan HRADECKÝ, Josef ZEHNÁLEK, Ladislav HAVEL, Libuše TRNKOVÁ a René KIZEK. Comparison of spectrophotometry to electrochemical methods of urease determination. In \textit{Chemické zvesti}. Slovenská republika: Institute of Chemistry Slovak Acedemy of Science, Bratislava, Slovak Chemical Society, Bratislava, 2007, s.~169-169. ISSN~1336-7242.
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