| HORNA, Aleš, Veronika ŠUPÁLKOVÁ, Karel STEJSKAL, Jiří BALOUN, Jitka PETRLOVÁ, Vojtěch ADAM, Ladislav HAVEL, Libuše TRNKOVÁ, René KIZEK a Josef ZEHNÁLEK. A new electrochemical assay for determination of phytochelatin synthase activity. In 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007. Belgické království: 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007, 2007, s. 911-911. |
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@inproceedings{746865,
author = {Horna, Aleš and Šupálková, Veronika and Stejskal, Karel and Baloun, Jiří and Petrlová, Jitka and Adam, Vojtěch and Havel, Ladislav and Trnková, Libuše and Kizek, René and Zehnálek, Josef},
address = {Belgické království},
booktitle = {31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007},
keywords = {Phytochelatin synthase; electrochemistry},
language = {eng},
location = {Belgické království},
pages = {911-911},
publisher = {31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007},
title = {A new electrochemical assay for determination of phytochelatin synthase activity},
year = {2007}
}
TY - JOUR
ID - 746865
AU - Horna, Aleš - Šupálková, Veronika - Stejskal, Karel - Baloun, Jiří - Petrlová, Jitka - Adam, Vojtěch - Havel, Ladislav - Trnková, Libuše - Kizek, René - Zehnálek, Josef
PY - 2007
TI - A new electrochemical assay for determination of phytochelatin synthase activity
PB - 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007
CY - Belgické království
KW - Phytochelatin synthase
KW - electrochemistry
N2 - Plants respond to heavy metal toxicity in a variety of different ways such as immobilization, exclusion, chelation and compartmentalization of the metal ions, and the expression of more general stress response mechanisms e.g. ethylene and stress peptides. One of the most studied plant stress peptides are phytochelatins participating in the detoxification of heavy metals. The synthesis of phytochelatins is catalyzed by phytochelatin synthase (PCS). The possible way of the study of PCS activity in the plant cell exposed by heavy metal is determination of increasing phytochelatins concentration. The main aim of the present work was to suggest a new tool (high performance liquid chromatography coupled with twelve channel electrochemical detector) for the determination of the phytochelatin synthase activity.
ER -
HORNA, Aleš, Veronika ŠUPÁLKOVÁ, Karel STEJSKAL, Jiří BALOUN, Jitka PETRLOVÁ, Vojtěch ADAM, Ladislav HAVEL, Libuše TRNKOVÁ, René KIZEK a Josef ZEHNÁLEK. A new electrochemical assay for determination of phytochelatin synthase activity. In \textit{31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007}. Belgické království: 31st International Symposium on High Performance Liquid Phase Separations and Related Techniques, HPLC 2007, 2007, s.~911-911.
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